Protein Info for PFLU_RS03240 in Pseudomonas fluorescens SBW25-INTG

Annotation: copper-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 733 transmembrane" amino acids 91 to 109 (19 residues), see Phobius details amino acids 121 to 141 (21 residues), see Phobius details amino acids 153 to 173 (21 residues), see Phobius details amino acids 181 to 200 (20 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details amino acids 364 to 388 (25 residues), see Phobius details amino acids 678 to 696 (19 residues), see Phobius details amino acids 702 to 720 (19 residues), see Phobius details PF00403: HMA" amino acids 10 to 68 (59 residues), 59.6 bits, see alignment 4.9e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 135 to 721 (587 residues), 593.2 bits, see alignment E=1.2e-181 TIGR01525: heavy metal translocating P-type ATPase" amino acids 153 to 720 (568 residues), 627.5 bits, see alignment E=5.1e-192 TIGR01494: HAD ATPase, P-type, family IC" amino acids 191 to 698 (508 residues), 285.2 bits, see alignment E=1.5e-88 PF00122: E1-E2_ATPase" amino acids 219 to 398 (180 residues), 187.2 bits, see alignment E=3.1e-59 PF00702: Hydrolase" amino acids 415 to 632 (218 residues), 113.7 bits, see alignment E=2.2e-36

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to pfs:PFLU0658)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K834 at UniProt or InterPro

Protein Sequence (733 amino acids)

>PFLU_RS03240 copper-translocating P-type ATPase (Pseudomonas fluorescens SBW25-INTG)
MNGSTTFDLPISGMTCASCAGRVERALGKVPGVQSVSVNLANERAHVEVLGQMDPGVLIA
AVDKAGYTASLPQSESTTDANQAQRLHHERWALLLAILLALPLVLPMLVEPFGLHWMLPA
WAQFALATPVQFIFGARFYIAAWKAVRAGAGNMDLLVAIGTSAGYGLSIYEWLTAHPGMA
PHLYFEASAVVIALVLLGKYLESRAKRQTASAIRALEALRPERAIRVLDGREEDVAINAL
KLNDLVLVKPGERFPVDGEVVEGQSHADEALISGESLPVPKQPGDNVTGGAINGEGRLLV
RTLALGAESVLARIIRLVEDAQSAKAPIQKLVDRVSQVFVPTVLVLALVTLVGWWLYGAP
LETAIINAVAVLVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALERAHAVSAVVFD
KTGTLTSGTPRIAHLAAVDGNEALLLQQAGALQRGSEHPLAKAVLDACNEKGLTVADVTA
SQSLTGRGIAGTLDGRPLALGNRRLLEESGLSAGDLADSAKSWEAEGRTLSWLIEQGAQP
RVLGLFAFGDTLKPGALEAVEQLKAQHISSHLLTGDNRGSARVVAEALGIDDVHAEVLPA
DKAATVVALKKTGVVAMVGDGINDAPALAAADIGIAMGGGTDVAMHAAGITLMRGDPRLV
PAALEISRKTYAKIRQNLFWAFVYNLIGIPLAAFGLLNPVLAGAAMALSSVSVVSNALLL
KTWKPKNLEDQRP