Protein Info for PFLU_RS02990 in Pseudomonas fluorescens SBW25-INTG
Annotation: precorrin-3B C(17)-methyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K13541, cobalamin biosynthesis protein CbiG / precorrin-3B C17-methyltransferase [EC: 2.1.1.131] (inferred from 100% identity to pfs:PFLU0607)Predicted SEED Role
"Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (12/15 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (27/36 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.1.131
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K6D5 at UniProt or InterPro
Protein Sequence (545 amino acids)
>PFLU_RS02990 precorrin-3B C(17)-methyltransferase (Pseudomonas fluorescens SBW25-INTG) MSPAIVILGQGSLATARKIQQVYPGALIHGLAGRVEGADQTYSEFGATLRQLYQHGTPLI ALCAAGIVIRTLAPLLLEKGEEPAVLAVAEDGSAVVPLLGGLGGVNVMARDIAAALDVAA AITTSGELRFGTCLLNPPKGYELADLELGKRFVSDLLAGERVRIEGAAPWLDQANLPQDQ QARLAIHVGSAERVPAANELLIYPKNVSVTCQPGAQLAQRVRTALHEAGIAVQSLACLLA SDTQMAEASLHEAALELGVPLRFVSASQEADIVIDVAEQPLDLSRVGRLRGRLAVIGLGP GAAELMVPAVKAELARCTDVLGYETYVRMAGPFRDDQVQHCTDNREEMQRARHAFELAAQ GRSVVVVSSGDPGVFAMAAAVIEALHASSDPAWHQVDLQILPGVSASLATAAQAGAPLGH DFCVMSLSDNLKPWSIIEKRLDLASQADLALAFYNPISRSRPWQLGRALEIVALHRTPQT PVVLGRDIGRPGQTLRVTTLGQLTPEQVDMRTMVLIGSSTTCTFPRAGGGEWVYTPRWYG EKPAS