Protein Info for PFLU_RS02975 in Pseudomonas fluorescens SBW25-INTG

Annotation: precorrin-3B synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR02435: precorrin-3B synthase" amino acids 30 to 407 (378 residues), 384.5 bits, see alignment E=2.7e-119 PF03460: NIR_SIR_ferr" amino acids 48 to 101 (54 residues), 41.2 bits, see alignment 1.1e-14 amino acids 272 to 326 (55 residues), 53.8 bits, see alignment 1.3e-18 PF01077: NIR_SIR" amino acids 114 to 238 (125 residues), 63.9 bits, see alignment E=1.2e-21

Best Hits

KEGG orthology group: K02229, precorrin-3B synthase [EC: 1.14.13.83] (inferred from 100% identity to pfs:PFLU0604)

Predicted SEED Role

"Cobalamin biosynthesis protein CobG" in subsystem Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.83

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6D2 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PFLU_RS02975 precorrin-3B synthase (Pseudomonas fluorescens SBW25-INTG)
MPPDKAGIIPRLYLQTGSPVNPTPAVNTLRPSACPGLLRIVQALDGGICRIKLAGGSITA
AQARAVAGAAQAYAGGVIEATNRANLQIRGIGAEEDALIARLLAADLGPRNAAGDDVRNL
MLSPSAGIDPQMLFDTRPLADEILATLQTHPRFHQLSAKFAVQLDGGEALAMLEHHHDLW
LSAFVRNGETLLAFGLAGCPGLDAPLAAVPLEQGHALVVAVLEAFLDLATPEHTRMRHLP
VDNLLSRLHLKLLPVDGFNRPPSGALLHLGTYPQAQKNQSYVAAIAPLGRLDSAMLTGVA
QLASEYGDGTLRFTPWQGVLLPNVEKPHAVTERLAQLGFLCSADQPLARMTACTGSSGCG
KGLADTKADAQQLAALDPGVDVHVSGCPRSCAAAHVAPVTLLAVSPGHYDLYFRDAAHPG
FGRLHARTLSIEATGALLRARPRSNTDD