Protein Info for PFLU_RS02735 in Pseudomonas fluorescens SBW25-INTG

Annotation: high-affinity branched-chain amino acid ABC transporter permease LivM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 425 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 47 to 65 (19 residues), see Phobius details amino acids 95 to 113 (19 residues), see Phobius details amino acids 119 to 141 (23 residues), see Phobius details amino acids 147 to 166 (20 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details amino acids 263 to 282 (20 residues), see Phobius details amino acids 314 to 337 (24 residues), see Phobius details amino acids 352 to 377 (26 residues), see Phobius details amino acids 389 to 406 (18 residues), see Phobius details PF11862: DUF3382" amino acids 12 to 109 (98 residues), 71.4 bits, see alignment E=6.1e-24 PF02653: BPD_transp_2" amino acids 117 to 402 (286 residues), 166 bits, see alignment E=9.7e-53

Best Hits

KEGG orthology group: K01998, branched-chain amino acid transport system permease protein (inferred from 100% identity to pfs:PFLU0554)

Predicted SEED Role

"Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4K6 at UniProt or InterPro

Protein Sequence (425 amino acids)

>PFLU_RS02735 high-affinity branched-chain amino acid ABC transporter permease LivM (Pseudomonas fluorescens SBW25-INTG)
MSAAKPIDIKKSVVDTVLAGLISLIVFGPIVGVVLDGYSFNLEPARVGLLVAIVMVGRFA
MSLFLQTPKGLKILQGFETSGSGVHVLAPDYKSRLRWIIPALIVIAIVFPIFANKYLLTV
VILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLALGYQYLGLGFWTVLPLAAIA
AALAGCILGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWLSFTGGPNGMPVPSPTFLGLEF
GKRAKDGGIPFHEFFGIDYNPNIKFLFIYIVLFLVVLAVLYIKHRLTRMPVGRAWEALRE
DEIACRSMGLNHVLVKLSAFTIGASTAGLAGVFFASYQGFVNPSSFTFFESALILAIVVL
GGMGSTVGVVIAAFVLTVAPELLRSFSEYRVLLFGVLMVVMMIWRPRGLIRISRTGVTPR
KGVAP