Protein Info for PFLU_RS02515 in Pseudomonas fluorescens SBW25-INTG

Annotation: flagellar motor protein MotB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 transmembrane" amino acids 21 to 45 (25 residues), see Phobius details PF13677: MotB_plug" amino acids 16 to 63 (48 residues), 66 bits, see alignment 1.8e-22 PF00691: OmpA" amino acids 166 to 256 (91 residues), 51.8 bits, see alignment E=8.6e-18

Best Hits

KEGG orthology group: K02557, chemotaxis protein MotB (inferred from 100% identity to pfs:PFLU0509)

Predicted SEED Role

"Flagellar motor rotation protein MotB" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDV2 at UniProt or InterPro

Protein Sequence (339 amino acids)

>PFLU_RS02515 flagellar motor protein MotB (Pseudomonas fluorescens SBW25-INTG)
MENNQPIIIKRVKRFAAGHHGGAWKIAFADFATAMMAFFLVLWLMSTATPEQKIAIAGYF
KDPIGFSESGTPFVIDLGGSPQLAPERTINPEVKTEAPQEKIPIERDTVEAMAEQVEQER
LELLLQELQTKVEENPQLLKFKDQISFEITPDGLRIQITDAANRPMFDSGSARLKPYFED
ILLAMADTIKAVPNKISISGHTDAKPYAGQGDFGNWELSANRANAARRALVAGSYPDPQV
ARVVGFASSQLFDHKDPFNPINRRIDIVVLTKKAQRAIEGDQQPAPPPTPGAGAPGEVPT
DPNAIPPGQEPLPAHELRQKLNLFDEGGVKDPTVPKAGS