Protein Info for PFLU_RS02430 in Pseudomonas fluorescens SBW25-INTG

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 transmembrane" amino acids 478 to 496 (19 residues), see Phobius details PF13667: ThiC-associated" amino acids 29 to 106 (78 residues), 105.8 bits, see alignment E=8.6e-35 TIGR00190: phosphomethylpyrimidine synthase" amino acids 145 to 597 (453 residues), 718 bits, see alignment E=1.7e-220 PF01964: ThiC_Rad_SAM" amino acids 146 to 595 (450 residues), 617.8 bits, see alignment E=9.4e-190

Best Hits

Swiss-Prot: 95% identical to THIC_PSESM: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to pfs:PFLU0492)

MetaCyc: 75% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDL3 at UniProt or InterPro

Protein Sequence (634 amino acids)

>PFLU_RS02430 phosphomethylpyrimidine synthase ThiC (Pseudomonas fluorescens SBW25-INTG)
MSTEIKSTKLKNTVHLSESAKVDSGSVQPFTRSQKIYVQGTRPDIRVPMREISLDVTPTD
FGGEINAPVVVYDTSGPYTDPNVIIDVRKGLGDVRSPWIEVRGDTERLSGLSSHFGQQRL
SDAELTALRFAHVKNPRRAKAGANVTQMHYARKGIITAEMEYVAIRENMKLEEARASGLL
DQQHAGHSFGASVPKIITPEFVREEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIG
NSALGSSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALE
KVGGAAEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAK
WCLAHHKENFTYTHFDEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTK
IAWKHDVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIG
AAMIGWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALS
KARFEFRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYA
ANQRIEAVDVEVAKGLAEQAERFKQEGSQLYKKV