Protein Info for PFLU_RS02280 in Pseudomonas fluorescens SBW25-INTG

Annotation: bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 979 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF03710: GlnE" amino acids 46 to 294 (249 residues), 285.8 bits, see alignment E=3e-89 amino acids 574 to 827 (254 residues), 313.5 bits, see alignment E=1.1e-97 PF08335: GlnD_UR_UTase" amino acids 315 to 453 (139 residues), 109.7 bits, see alignment E=1.3e-35 amino acids 872 to 947 (76 residues), 30.3 bits, see alignment 4e-11

Best Hits

Swiss-Prot: 82% identical to GLNE_PSESM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to pfs:PFLU0461)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCP5 at UniProt or InterPro

Protein Sequence (979 amino acids)

>PFLU_RS02280 bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase (Pseudomonas fluorescens SBW25-INTG)
MSLPTLAELPAILLPKAQRAEQSFRDAVAALDDDHGLSAWTPQRWADFTRVCAASDFVIE
QSVRDPLMLLELVAWGELDRGFAPGELCGQIAGAVQQAETEDQLGRVLRRQRTRQQVRII
WRDLTRQADLVQTCRDLSDMADASIDQAYQWLYLRHCVQFGTPTGRRSGEPQHMVILGMG
KLGAVELNLSSDIDLIFAYPEGGETVGVKRALDNQEFFIRLGQKLIKALDPMTVDGFVFR
VDMRLRPYGSAGALVLSFNALEQYYQDQGRDWERYAMIKARVVAGDQVAGAQLLDMLRPF
VYRRYLDFSAIEALRTMKQLIQQEVRRKGMADNIKLGSGGIREVEFIAQAFQLIHGGRDL
SLQQRPLLKVLGTLEGQGYLPPAVIAELRNGYEFLRYTEHAIQAIADRQTQMLPDDPEDQ
ARIAFMLGFADWAAFHERLMYWRGRVDWHFRQVIADPDEEEGEESELVVGGEWLPLWEES
QDDEAACRQLAEGGFSDATKALKALTGLRNSPQLRAMQRLGRERLDAFIPRLLAQAVEHA
NPDLVLERVLPLVEAVARRSAYLVLLTENPDALRRLLTLCAASPWIAEQITRFPLLLDEL
LNEGRLFKPPLAPELAAELRERLTRIPEDDLEQQMEALRHFKLAHRLRVAASEIAGSLPL
MKVSDYLTWLAEAILEQVLALAWRQTVARHGSPQRLDGTLCDPGFIIVGYGKVGGIELGH
GSDLDLVFIHDGDPQAETDGAKPIDGAQFFTRLGQRIIHLLTTQTNSGQLYEVDMRLRPS
GASGLLVSSLGAFDRYQQNEAWTWEHQALIRARVLVGSQDVGQAFEQVRAKVLGRERDLA
KLRQEVSEMRAKMRDNLGTKATTAGTGANAFEATAAFDLKQDAGGIVDIEFMVQYAALAW
SAQHPSLLRYTDNIRILEGLEQVGLMPAADAHLLREVYKAYRSAAHRQALQNEAGTVAGD
QFADERRQVMRIWQALGLS