Protein Info for PFLU_RS02230 in Pseudomonas fluorescens SBW25-INTG

Annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 72 bits, see alignment (E = 5.3e-24)
• PF18327: PRODH
  • amino acids 87 to 134 (48 residues), 63.7 bits, see alignment (E = 3.7e-21)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 146 to 257 (112 residues), 151.7 bits, E=2.6e-48
• PF01619: Pro_dh
  • amino acids 267 to 567 (301 residues), 357.3 bits, E=1.7e-110
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 608 to 1108 (501 residues), 806.6 bits, E=3.8e-247
• PF00171: Aldedh
  • amino acids 655 to 1101 (447 residues), 381.9 bits, E=9.2e-118

Best Hits

Swiss-Prot: 74% identical to PUTA_KLEAE: Bifunctional protein PutA (putA) from Klebsiella aerogenes

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 68% identity to aav:Aave_3321)

MetaCyc: 74% identical to trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Salmonella enterica enterica serovar Typhimurium)
RXN-14903 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-N^δ-acetylornithine biosynthesis (6/7 steps found)
• L-arginine degradation I (arginase pathway) (3/3 steps found)
• L-proline degradation I (3/3 steps found)
• proline to cytochrome bo oxidase electron transfer (1/2 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Sequence Analysis Tools

See C3KCN5 at UniProt or InterPro

Protein Sequence (1317 amino acids)

>PFLU_RS02230 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Pseudomonas fluorescens SBW25-INTG)
MATTTLGVKLDDPTRERLKAAATSIDRTPHWLIKQAIFNYLEKLEGGATLTELNGLSSNG
TDDAGDVQTDHAHQCFLEFAESILPQSVLRASITAAYRRPEPEVVPMLIEQARLPAPMAE
ATNKLAASIAEKLRNQKSAGGRAGIVQGLLQEFSLSSQEGVALMCLAEALLRIPDKGTRD
ALIRDKISTGNWQPHLGNSPSLFVNAATWGLLLTGKLVATHNEAGLTSSLSRIIGKSGEP
MIRKGVDMAMRLMGEQFVTGETIAEALANASKFEAKGFRYSYDMLGEAALTERDAQKYLA
SYEQAIHSIGKASHGRGIYEGPGISIKLSALHPRYSRAQYERVMDELYPRLLSLTLLAKQ
YDIGLNIDAEEADRLELSLDLLERLCFEPQLTGWNGIGFVIQAYQKRCPYVIDYVIDLAR
RSRHRLMIRLVKGAYWDSEIKRAQVEGLEGYPVYTRKVYTDVSYIACARKLLSVPEVIYP
QFATHNAHTLSAIYHIAGQNYYPGQYEFQCLHGMGEPLYEQVVGKVSDGKLNRPCRVYAP
VGTHETLLAYLVRRLLENGANTSFVNRIADQSISIQELVADPVASIEQMATLEGGFGLPH
PRIPLPRDLYGRDRANSAGIDLANEHRLASLSCALLATAHNNWKAAPMLGCAASEQAAAP
VLNPSDLRDVVGHVQEATVEDVDNAIQCAISAGPIWQATPPAERAAILERAADLMEGEIQ
PLMGLLAREAGKTFANAIAEVREAVDFLRYYAVQARNDFTNDAHRPLGPVVCISPWNFPL
AIFSGQVAAALAAGNPVLAKPAEQTPLVAAQAVRILLEAGIPEGVLQLLPGQGETVGARL
VGDDRVKGVMFTGSTEVARLLQRNVAGRLDAQGRPIPLIAETGGQNAMIVDSSALTEQVV
IDVVSSAFDSAGQRCSALRVLCLQEDSADRVIEMLKGAMAECRLGNPERLSVDIGPVIDA
EAKAGIEKHIQAMRDKGRNVYQVAIADGEEIKRGTFVTPTLIELESFDELQREIFGPVLH
VVRYKRKEIDQLIGQINASGYGLTLGVHTRIDETIAKVIDNVNAGNVYVNRNIVGAVVGV
QPFGGEGLSGTGPKAGGPLYLYRLLSTRPTDAIEQSFVRGDALAAPDVRLRDAMSQPLTA
LNTWADSNKLSELSALCSQFAAQSQSGITRQLAGPTGERNSYAILPREHVLCLADVEGDL
LTQLAAVLAVGGSAVWPETDLTKALFPRLPKEIQAKIKRVADWTKDEVVFDAVLHHGDSD
QLRAVCQQVAQRGGAIVGVHGLSQGETAIALERLVIERALSVNTAAAGGNASLMTIG