Protein Info for PFLU_RS01860 in Pseudomonas fluorescens SBW25-INTG

Annotation: amino acid ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00005: ABC_tran
  • amino acids 22 to 169 (148 residues), 136.7 bits, E=1.3e-43
• PF03215: Rad17
  • amino acids 28 to 129 (102 residues), 24.8 bits, E=3e-09

Best Hits

Swiss-Prot: 61% identical to GLNQ_BACSU: Glutamine transport ATP-binding protein GlnQ (glnQ) from Bacillus subtilis (strain 168)

KEGG orthology group: K10041, putative glutamine transport system ATP-binding protein [EC: 3.6.3.-] (inferred from 100% identity to pfs:PFLU0378)

MetaCyc: 62% identical to glutamate/aspartate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-13-RXN [EC: 7.4.2.1]; 7.4.2.1 [EC: 7.4.2.1]

Predicted SEED Role

"Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.4.2.1

Sequence Analysis Tools

See C3K820 at UniProt or InterPro

Protein Sequence (244 amino acids)

>PFLU_RS01860 amino acid ABC transporter ATP-binding protein (Pseudomonas fluorescens SBW25-INTG)
MIEVRDLVKVFDTRGHVVRAVDHVTTRVAKGEVLVVIGPSGSGKSTFLRCLNGLEEFDSG
SVSIDGLQLADPKTDVNAYRREVGMVFQHFNLFPHMTVLENLCLAQKVVRKRGKQEREAK
ALALLEKVGIAQKANEFPSRLSGGQQQRVAIARALAMEPKVMLFDEPTSALDPEMVGEVL
DVMKTLALEGMTMVCVTHEMGFAREVADRVLFFDHGKLLEDAAPAEFFDSPKDPRAQAFL
RQVL