Protein Info for PFLU_RS01775 in Pseudomonas fluorescens SBW25-INTG

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 TIGR01228: urocanate hydratase" amino acids 21 to 564 (544 residues), 1019.6 bits, see alignment E=0 PF17391: Urocanase_N" amino acids 22 to 148 (127 residues), 206.4 bits, see alignment E=1.6e-65 PF01175: Urocanase" amino acids 151 to 359 (209 residues), 296.2 bits, see alignment E=2.1e-92 PF17392: Urocanase_C" amino acids 362 to 556 (195 residues), 311.2 bits, see alignment E=3.6e-97

Best Hits

Swiss-Prot: 100% identical to HUTU_PSEFS: Urocanate hydratase (hutU) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to pfs:PFLU0361)

MetaCyc: 92% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K803 at UniProt or InterPro

Protein Sequence (566 amino acids)

>PFLU_RS01775 urocanate hydratase (Pseudomonas fluorescens SBW25-INTG)
MRVDRRGVLAVTKPTKYRDVEIRAAHGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELV
VYGGIGRAARNWECYDQIVESLTHLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVP
HWASWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYDSNLKGRWV
LTAGLGGMGGAQPLAATLAGACSLNIECQQVSIDFRLNSRYVDEQATDLDDALARIAKYT
QEGKAISIALLGNAAEILPELVKRGVRPDMVTDQTSAHDPLNGYLPAGWTWDEYRARAKT
EPAAVIKAAKQSMAVHVKAMLAFQKQGIPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYI
RPLFCRGVGPFRWAALSGDPQDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARI
CWVGLGQRAKLGLAFNEMVRSGELSAPVVIGRDHLDSGSVASPNRETEAMQDGSDAVSDW
PLLNALLNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPATGV
MRHADAGYQIAIDCAKEQGLNLPMIK