Protein Info for PFLU_RS01730 in Pseudomonas fluorescens SBW25-INTG

Annotation: glycogen/starch/alpha-glucan phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 13 to 813 (801 residues), 1097.3 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 100 to 813 (714 residues), 1004.3 bits, see alignment E=1.6e-306

Best Hits

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to pfs:PFLU0352)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7D3 at UniProt or InterPro

Protein Sequence (816 amino acids)

>PFLU_RS01730 glycogen/starch/alpha-glucan phosphorylase (Pseudomonas fluorescens SBW25-INTG)
MSQEPLAREAEVAAFRDAVLTKLTYAVGKDPDHAFDHDWFEAIALAARDQMVDHWMDHTR
RIYRKGQKRVYYLSLEFLIGRLLYDSLSNLGVLEIAREALSELGVDLERIRLLEPDAALG
NGGLGRLAACFMESMSTLGIAGHGYGIRYEHGLFRQAIVDGWQQEQTERWLDFGNPWEFE
RAEVIYPIGFGGSVETLPDASGKQIQVWSPSETVRAVAYDTPVVGWRGASVNTLRLWRAR
AVEDLHLERFNAGDHLGAVAEVARAESISRVLYPADSTEAGQELRLRQEYFFVSASLQDL
LRRHKNMHGSVLSLGEHAAIQLNDTHPSIAVAELMRQLVDLHDIPWEAAWDVTVETLSYT
NHTLLPEALETWPVGLMERMLPRHMQIIYLINAQHIDSLRAKGIHDFDVLRAVSLIEEDN
GRRVRMGNLAFLGSHSVNGVSGLHTQLMRSTVFSELHKLYPDRINNKTNGITFRRWLYQA
NPKLTQMLVEALGPDILDNMETRLGELETFAEKQTFRKAFADQRLHSKRALADIIHERLG
IAINPAAMFDVQVKRIHEYKRQLLNLLHTVALYQAIRAEPGTDWVPRVKIFAGKAAASYH
QAKLIIKLTNDIARTVNNDPTVRGLLKVVFLPNYNVSLAESIIPAADLSEQISTAGFEAS
GTSNMKFGLNGALTIGTMDGANVEMHERVGADHMFIFGLSAQQVEARKHAGEFNAGPDIA
ASHRLNDVLQAIRGGVFSPDDPGRYVGLIDGLIDYDRFLVCADFDSYWDAQARVEAHWHD
SKAWWRSAVLNTARMGWFSSDRTIREYATEIWKALD