Protein Info for PFLU_RS01685 in Pseudomonas fluorescens SBW25-INTG

Annotation: nitrogen regulation protein NR(I)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 TIGR01818: nitrogen regulation protein NR(I)" amino acids 7 to 463 (457 residues), 799.3 bits, see alignment E=5.4e-245 PF00072: Response_reg" amino acids 7 to 116 (110 residues), 105 bits, see alignment E=6.6e-34 PF00158: Sigma54_activat" amino acids 141 to 308 (168 residues), 241.5 bits, see alignment E=1e-75 PF14532: Sigma54_activ_2" amino acids 142 to 311 (170 residues), 81 bits, see alignment E=2.5e-26 PF07728: AAA_5" amino acids 165 to 283 (119 residues), 31.5 bits, see alignment E=4.1e-11 PF02954: HTH_8" amino acids 423 to 463 (41 residues), 48.1 bits, see alignment 2e-16

Best Hits

Swiss-Prot: 70% identical to NTRC_SALTY: DNA-binding transcriptional regulator NtrC (glnG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K07712, two-component system, NtrC family, nitrogen regulation response regulator GlnG (inferred from 100% identity to pfs:PFLU0343)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7C4 at UniProt or InterPro

Protein Sequence (478 amino acids)

>PFLU_RS01685 nitrogen regulation protein NR(I) (Pseudomonas fluorescens SBW25-INTG)
MSRSETVWIVDDDRSIRWVLEKALQQEGMTTQSFDSADGVMSRLARQQPDVIISDIRMPG
ASGLDLLARIREQHPRLPVIIMTAHSDLDSAVASYQGGAFEYLPKPFDVDEAVALVKRAN
QHAQEQQNQEAPPALTRTPEIIGEAPAMQEVFRAIGRLSHSNITVLINGESGTGKELVAH
ALHRHSPRAASPFIALNMAAIPKDLMESELFGHEKGAFTGAANLRRGRFEQADGGTLFLD
EIGDMPADTQTRLLRVLADGEFYRVGGHTPVKVDVRIIAATHQNLETLVHAGKFREDLFH
RLNVIRIHIPRMSDRREDIPTLARHFLSRAAQELAVEPKLLKSETEEYLKNLPWPGNVRQ
LENTCRWITVMASGREVHISDLPPELLSLPQDSAPVTNWEQALRQWADQALARGQSNLLD
SAVPAFERIMIETALKHTAGRRRDAAVLLGWGRNTLTRKIKELGMKVDGGDDDEGDEG