Protein Info for PFLU_RS01540 in Pseudomonas fluorescens SBW25-INTG

Annotation: ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 PF00005: ABC_tran" amino acids 23 to 182 (160 residues), 123 bits, see alignment E=1.5e-39 PF13304: AAA_21" amino acids 111 to 212 (102 residues), 31.2 bits, see alignment E=2.3e-11

Best Hits

Swiss-Prot: 66% identical to AOTP_PSEAE: Arginine/ornithine transport ATP-binding protein AotP (aotP) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02028, polar amino acid transport system ATP-binding protein [EC: 3.6.3.21] (inferred from 100% identity to pfs:PFLU0314)

Predicted SEED Role

"Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1)" in subsystem Arginine and Ornithine Degradation (TC 3.A.1.3.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.21

Use Curated BLAST to search for 3.6.3.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6C3 at UniProt or InterPro

Protein Sequence (257 amino acids)

>PFLU_RS01540 ABC transporter ATP-binding protein (Pseudomonas fluorescens SBW25-INTG)
MAEATPALEIRNLHKRYGELEVLKGISLTARDGDVISILGSSGSGKSTFLRCINLLENPH
QGQILVAGEELKLKAAKNGELMAADGKQINRLRSEIGFVFQNFNLWPHMSILDNIIEAPR
RVLGQSKAEAIEVAEALLDKVGIADKRHAYPAQLSGGQQQRAAIARTLAMQPKVILFDEP
TSALDPEMVQEVLNVIRALAEEGRTMLLVTHEMGFARQVSSEVVFLHQGLVEEQGSPQQV
FENPLSARCKQFMSSNR