Protein Info for PFLU_RS00540 in Pseudomonas fluorescens SBW25-INTG

Annotation: acetyl-CoA hydrolase/transferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 PF02550: AcetylCoA_hydro" amino acids 12 to 215 (204 residues), 100.6 bits, see alignment E=2.5e-32 TIGR03458: succinate CoA transferase" amino acids 13 to 494 (482 residues), 772.7 bits, see alignment E=6.8e-237 PF13336: AcetylCoA_hyd_C" amino acids 323 to 463 (141 residues), 138.9 bits, see alignment E=2.7e-44

Best Hits

KEGG orthology group: K01067, acetyl-CoA hydrolase [EC: 3.1.2.1] (inferred from 100% identity to pfs:PFLU0110)

Predicted SEED Role

"Propionyl-CoA:succinyl-CoA transferase"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7Y8 at UniProt or InterPro

Protein Sequence (497 amino acids)

>PFLU_RS00540 acetyl-CoA hydrolase/transferase family protein (Pseudomonas fluorescens SBW25-INTG)
MYRDRIRLPSLLNKVMSAADAAALIEDGMTVGMSGFTRAGEAKAVPHALAERAKTSPLKI
TLMTGASLGNDLDKQLTEAGVLSRRMPFQVDSTLRKAINAGEVMFIDQHLSETVEQLRNN
QLKLPDIAVIEAVAITEQGHIVPTTSVGNSASFAIFAKQVIVEINMAHNPNLEGLHDIYI
PTYRPTRTPIPLVKVDDRIGSTAIPIPPEKIVAIVITNQADSASTVTPPDSDTQGIANHL
INFLKQEVDAGRMTNKLGPLQAGIGNIANAVMCGLIDSPFEDLTMYSEVLQDSTFDLIDA
GKLSFASGSSITLSERRNADVFGNLEHYKDKLVLRPQEISNHPEVVRRLGIIGINTALEF
DIYGNVNSTHICGTRMMNGIGGSGDFARNAHLAIFVTKSIAKGGAISSVVPMVSHVDHTE
HDVDILVTEVGLADLRGLAPRERARVIIDNCVHPAYRDALNSYFDAACAIGGHTPHILRE
ALSWHINLEETGHMLKA