Protein Info for PFLU_RS00440 in Pseudomonas fluorescens SBW25-INTG

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 PF00072: Response_reg" amino acids 11 to 119 (109 residues), 101.6 bits, see alignment E=1e-32 PF00158: Sigma54_activat" amino acids 150 to 314 (165 residues), 214.6 bits, see alignment E=2.7e-67 PF14532: Sigma54_activ_2" amino acids 158 to 318 (161 residues), 53.6 bits, see alignment E=1.1e-17 PF07728: AAA_5" amino acids 170 to 289 (120 residues), 26.2 bits, see alignment E=2.5e-09 PF00004: AAA" amino acids 171 to 302 (132 residues), 23.5 bits, see alignment E=2.2e-08 PF02954: HTH_8" amino acids 406 to 446 (41 residues), 37 bits, see alignment 8.1e-13

Best Hits

Swiss-Prot: 95% identical to ALGB_PSESM: Alginate biosynthesis transcriptional regulatory protein AlgB (algB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K11384, two-component system, NtrC family, response regulator AlgB (inferred from 100% identity to pfs:PFLU0088)

Predicted SEED Role

"alginate biosynthesis transcriptional regulatory protein AlgB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6T8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PFLU_RS00440 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25-INTG)
MESAKELQGRILLVDDESAILRTFRYCLEDEGYTVATANSAAQADTLMQRQVFDLCFLDL
RLGEDNGLDVLAQMRIQAPWMRVVIVTAHSAVDTAVDAIQAGAADYLVKPCSPDQLRLAT
AKQLEVRQLSARLEALEGAVRQPKDGLDSHSPSMMVVLETARQVAGTDANILILGESGTG
KGELARAIHGWSKRSKKSCVTINCPSLTAELMESELFGHSRGAFTGASESTLGRVNQADG
GTLFLDEIGDFPLTLQPKLLRFIQDKEYERVGDPVTRRADVRILAATNLNLEDMVRDGRF
REDLLYRLNVITLHLPPLRERSEDILTLADRFLARFVKDYARPARGFSDEAREALLNYRW
PGNIRELRNVVERASIICPQEKVEISHLGMAEQPTNNAPRIGAALSLDELEKAHIGAVLA
TSDTLDQAARTLGIDASTLYRKRKQYNL