Protein Info for MPMX20_04690 in Enterobacter sp. TBS_079

Annotation: Multifunctional conjugation protein TraI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1765 TIGR02760: conjugative transfer relaxase protein TraI" amino acids 1 to 1458 (1458 residues), 1110.3 bits, see alignment E=0 PF08751: TrwC" amino acids 11 to 279 (269 residues), 227.9 bits, see alignment E=7.1e-71 TIGR02686: conjugative relaxase domain" amino acids 12 to 285 (274 residues), 278.9 bits, see alignment E=5.5e-87 PF22232: TraI_hel_assoc_N" amino acids 450 to 547 (98 residues), 78.7 bits, see alignment 1.6e-25 PF18272: ssDNA_TraI_N" amino acids 586 to 637 (52 residues), 39.3 bits, see alignment (E = 1.7e-13) PF18340: TraI_2B" amino acids 644 to 723 (80 residues), 89.8 bits, see alignment 3e-29 PF13604: AAA_30" amino acids 971 to 1158 (188 residues), 122.6 bits, see alignment E=6e-39 PF13245: AAA_19" amino acids 975 to 1106 (132 residues), 51.9 bits, see alignment 3.2e-17 PF07057: TraI_C" amino acids 1470 to 1626 (157 residues), 116 bits, see alignment E=5e-37

Best Hits

Predicted SEED Role

"IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1765 amino acids)

>MPMX20_04690 Multifunctional conjugation protein TraI (Enterobacter sp. TBS_079)
MLSISSIKGDSAYYSHEDNYYVSGSLESRWLGDGAEKLGLKGEVANADMDAIRQGKLPDG
TDLSRMVNGVNKHRSGYDLTFSAPKSVSMMILIGGDTRLLDAWNRSVENGMKEVEKLISA
RITDGGKTGTVLTGNMVAALYNHDTSRALDPQIHTHALVFNTTFAEDKWRALASDTRMKT
GFGENLYALQVAIGNLVLQPFRQEAEKLGYETVTAGKNGLWELKDVPVAPFSTRSQAISE
AAGPDASKKSRDVAALDTRQAKAWAAPDLLKAEWRRRLTDEKFDIDNYIGQAQTRAEPPG
PVGGGTEDIRSTGQPDSASSAQISESDVQKAVSDTISALSEKKVQFTWSEMLAGTVSRLP
SASGLFEQARAGIDAAIEEQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVQEQTVLIF
PERAQEREMPAGDAVSVLTQDKSPVAILSGRGGAQTLRERTEDVAMMARSQGREVMVIAA
DGRSGQFLSESPHLAGHVMLRSQMNADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAG
AQLIFMDTENRQGTGNALSVLKEADIPQYRFYSTQLPEVRLVSEADKRSRYGQLAQEYVR
LSAEGRDVVAQVTGAREQQQLTEVIRDIRRDAGELDREQVTLGVLEPVWLDSKTRHQRDN
YRPGMVMEHWDAEKKTMTRHTIDRVAEATNSLVLQGEDGSRLTLKVTQVDGSWSLYRSRT
LDISEGDRVRALGRELKGAIKAKEQFTVAGLENGVVRLRSGDRELRLPTERAVKLAHDYV
EGTGAGTSASRTVLAAVGPRGLNKQTLNALAQSGNDIRIYTPLAPEQAARKVESVSAVRL
ASDQVRQSTGEANLDTAIQASRERLMSDAEQAVSLAIPRAQQGKVHLSEIALLSEAVKSG
QPLADVRTEIARQVNSGDLIQLDSVSGAGNRVLVPRVAYEMEKTIIRHIAEGKDAVQPLM
ALTPASVLAGLTAGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRP
QVIGLGPTHRAVHEMREAGVEARTLASFLSETRLAIQAGETPDFRNVLFLTDESSMVGNR
DMAELYQLVAAGGGRMVSSGDTAQLQAISTGLPFRLVQQRSAIDTVVMQEIVRQTPALRP
AIESIIAGQVDTSLRQVDDVSPQQVPRQEGAWVPGNSVMEIRAPKKDQEQDSPAVDEQTL
TPEQLSLVRTDIIEAIRDDWMGRTPEAQQQTLVVAELNADRHAINDAIHAARHEKGDTGA
EERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGES
VLISPQQNTAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDDGAIRFR
QGDQEKIVDPQAMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVT
LSRAKEHVQVYTDDLAGWSAGARHSNAGQTAHDLLHQKSDHESDTGNRLLATASRLDKTA
LGRRVLAENGLEGETMARFIAAGRKYPTPYVALPAWTRHGQEAGALLTEIRIEDDGMRVV
LSDESRLRGGEDAQFAGLQASRNGQTLIADSAEAALQLAQQNPESGVVIRLHGEERLLNA
ARLTGGRITEPDEVARTVRSVADAEAAAKAEDPITLPPDEQQKLAEAQEKAARELAEQAR
QERLPAVPGEESTRPEPLLSADEEGRLRDGTARGERELDEAIQEAVAEGRGIRQQVREQM
LRTEREWVVNVPEKDIELEKTLGGD