Protein Info for MPMX20_04515 in Enterobacter sp. TBS_079

Annotation: tRNA/tmRNA (uracil-C(5))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 TIGR02143: tRNA (uracil(54)-C(5))-methyltransferase" amino acids 10 to 365 (356 residues), 611.1 bits, see alignment E=2.8e-188 PF05958: tRNA_U5-meth_tr" amino acids 10 to 365 (356 residues), 629.3 bits, see alignment E=1.9e-193 PF13649: Methyltransf_25" amino acids 215 to 273 (59 residues), 27.4 bits, see alignment E=4.6e-10

Best Hits

Swiss-Prot: 94% identical to TRMA_ENT38: tRNA/tmRNA (uracil-C(5))-methyltransferase (trmA) from Enterobacter sp. (strain 638)

KEGG orthology group: K00557, tRNA (uracil-5-)-methyltransferase [EC: 2.1.1.35] (inferred from 98% identity to enc:ECL_05023)

MetaCyc: 92% identical to tRNA m5U54 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (uracil-5-)-methyltransferase. [EC: 2.1.1.35]

Predicted SEED Role

"tRNA (Uracil54-C5-)-methyltransferase (EC 2.1.1.35)" (EC 2.1.1.35)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.35

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (366 amino acids)

>MPMX20_04515 tRNA/tmRNA (uracil-C(5))-methyltransferase (Enterobacter sp. TBS_079)
MTPEHLPTEQYDAQLAEKVVRLQSMMTPFNAPVPEVFRSPVSHYRMRAEFRIWHDGDDLY
HIIFDQQTKSRIRVDSFPAASELINQLMTLMIDGVRNNPVLRHKLFQIDYLTTQSNQAIV
SLLYHKALNDEWREQAEALRDALRAQNINVHLIGRATKTKIMLDQDFIDERLSVAGKEMV
YRQVENSFTQPNAAMNVQMLDWALKATEGSKGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANHIDNVQIIRMSAEEFTQAMNGVRQFNRLEGIDLKSYQCETIFVDP
PRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQTHNVERLALFDQFPYTHHMECG
VLLTAK