Protein Info for MPMX20_04414 in Enterobacter sp. TBS_079

Annotation: putative cyclic di-GMP phosphodiesterase PdeK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 152 to 178 (27 residues), see Phobius details PF17154: GAPES3" amino acids 36 to 154 (119 residues), 158 bits, see alignment E=1.5e-50 PF00990: GGDEF" amino acids 247 to 394 (148 residues), 38.5 bits, see alignment E=1.6e-13 PF00563: EAL" amino acids 415 to 647 (233 residues), 219 bits, see alignment E=9.7e-69

Best Hits

Swiss-Prot: 78% identical to PDEK_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeK (pdeK) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to enc:ECL_04934)

MetaCyc: 78% identical to c-di-GMP phosphodiesterase PdeK (Escherichia coli K-12 substr. MG1655)
Cyclic-guanylate-specific phosphodiesterase. [EC: 3.1.4.52]

Predicted SEED Role

"Protein yhjK"

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.52

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (668 amino acids)

>MPMX20_04414 putative cyclic di-GMP phosphodiesterase PdeK (Enterobacter sp. TBS_079)
MRVSRSLTIKQMAMVSAVTMLFVLLFCVILLFHSVQQNRYNTASQLESIARSVRGPLSAS
ILKGDIPEAETILKRIQPAGVVSRADVVLPNQFQALRMSFIPERPVPMMVMRLFELPVQI
SLPVYSLERPANPQPLAYLVLQADSYRMYKFVMSWVVTLVTTCLLMTLILSVALTWCINR
LIVHPLRRIARELNTLSPQDQVGHQLPLPRLHHDDEIGMLVRSYNLNQQRMQRQQDELNS
SATRFPVSELPNKAFLMAMLEQTVARQQTTALMVIACETLQDTAGVLKENQREMLLLTLV
EKVKSVLAPRMVLTQVSGYDLVVIANGVKEPWHAITLGQQVLTVINERLPIQGIQLRPSA
SIGIAMYYGDLTAEQLYRRAFSAAFTARRKGKNQIQFFDPEQMERAQQRLTEESDILTAL
DNRQFALWLQPQVNLLTGEVISAEALLRVQQPDGSWELPEGLIERIESCGLMVTVGYWVL
EESCRQLAAWQERGVTLPLSVNLSALQLMHPTMVSEMLELIHRYRIKPDTLTLEVTESRR
IDDPNEAVAILKPLRNAGIRIALDDFGMGYAGLRQLQHMKTLPVDVLKIDKAFVEGLPED
SSMVQAIIQLARSLKLQLIAEGIETDAQRTWLAEAGVEGGQGFLFAPAVPSDVFEQRYLS
GGDNSANV