Protein Info for MPMX20_04400 in Enterobacter sp. TBS_079
Annotation: Cytoplasmic trehalase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to TREF_SALCH: Cytoplasmic trehalase (treF) from Salmonella choleraesuis (strain SC-B67)
KEGG orthology group: K01194, alpha,alpha-trehalase [EC: 3.2.1.28] (inferred from 98% identity to enc:ECL_04918)MetaCyc: 87% identical to cytoplasmic trehalase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalase. [EC: 3.2.1.28]
Predicted SEED Role
"Cytoplasmic trehalase (EC 3.2.1.28)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.28)
MetaCyc Pathways
- trehalose degradation II (cytosolic) (2/2 steps found)
- trehalose degradation VI (periplasmic) (2/2 steps found)
- chitin biosynthesis (6/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.28
Use Curated BLAST to search for 3.2.1.28
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (549 amino acids)
>MPMX20_04400 Cytoplasmic trehalase (Enterobacter sp. TBS_079) MFNQKLQAAENIEFEIAEELRYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYL ELFEHVQSTRLFADSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETYT TEYVSDPGLSLKEHIDSLWPVLTREPQDHIPWSSLLALPQAYIVPGGRFSETYYWDSYFS MLGLAESGRNDLLKCMADNFAWLIERYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR GAKRYLEHLKMEHAFWMDGAESLLLNQAYRSAVRMPDGSLLNRYWDDRDTPRDESWIEDV ETARHSGRPPNEVYRDLRAGAASGWDYSSRWLREPGRLASIRTTQFIPIDLNAFLFKLES AIANISASKGDKETAELFRQKASDRRAAVNRYLWDDENGCYRDYDWRREVMALFSAASIV PLYVGMATHEQAERLSDAVKARLLTPGGILATEYETGEQWDKPNGWAPLQWMAIQGFKQY GNDSLGDEIAWSWLQTVNHFYKAHHKLIEKYHIASSTPREGGGGEYPLQDGFGWTNGVVR RLIGLYGEP