Protein Info for MPMX20_04336 in Enterobacter sp. TBS_079

Annotation: Leu/Ile/Val-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13433: Peripla_BP_5" amino acids 23 to 335 (313 residues), 58.9 bits, see alignment E=7.5e-20 PF13458: Peripla_BP_6" amino acids 23 to 341 (319 residues), 178.3 bits, see alignment E=5.5e-56 PF01094: ANF_receptor" amino acids 45 to 355 (311 residues), 80.4 bits, see alignment E=2e-26

Best Hits

Swiss-Prot: 96% identical to LIVJ_ECO57: Leu/Ile/Val-binding protein (livJ) from Escherichia coli O157:H7

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 97% identity to enc:ECL_04820)

MetaCyc: 96% identical to branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-15-RXN [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]; 7.4.2.2 [EC: 7.4.2.2]

Predicted SEED Role

"High-affinity leucine-specific transport system, periplasmic binding protein LivK (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (364 amino acids)

>MPMX20_04336 Leu/Ile/Val-binding protein (Enterobacter sp. TBS_079)
MKGKALLAGCIALAFSTMAQADIKVAVVGAMSGPVAQYGDQEFTGAEQAVADINAKGGIK
GEKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCSSSTQPASDIYEDEGILMITPA
ATAPELTARGYKLTLRTTGLDSDQGPTAAKYILDNVKPKRIAIVHDKQQYGEGLARAVQD
GLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMGQILRQARAAGLKT
QFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKPIVDAIKAKKQDPSGAFVWTT
YAALQSLQAGLNQSADPAEIATWLKAHSVDTVMGPLSWDAKGDLKGFEFGVFDWHANGTA
TDAK