Protein Info for MPMX20_04273 in Enterobacter sp. TBS_079

Annotation: Tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 PF00579: tRNA-synt_1b" amino acids 3 to 285 (283 residues), 288.5 bits, see alignment E=2.9e-90 TIGR00233: tryptophan--tRNA ligase" amino acids 3 to 330 (328 residues), 386.4 bits, see alignment E=5.9e-120

Best Hits

Swiss-Prot: 94% identical to SYW_ECOL6: Tryptophan--tRNA ligase (trpS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 99% identity to enc:ECL_04746)

MetaCyc: 94% identical to tryptophan--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Tryptophan--tRNA ligase. [EC: 6.1.1.2]; 6.1.1.2 [EC: 6.1.1.2]

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.2

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>MPMX20_04273 Tryptophan--tRNA ligase (Enterobacter sp. TBS_079)
MTKPIVFSGAQPSGELTIGNYMGALRQWVSMQDDYHCIYCIVDLHAITARQDPEKLRKAT
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYSEN
INAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDIAQRFNALYGDVFKVPEPFIP
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKLKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSGVTGQSIPELEQHFEGKMYGHLKGEVADAVSGMLTELQERYHRYRNDEA
FLQKVMKEGAEKASARASETLKAVYQAIGFVGKP