Protein Info for MPMX20_03841 in Enterobacter sp. TBS_079
Annotation: Bifunctional glutathionylspermidine synthetase/amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 83% identical to GSP_ECOLI: Bifunctional glutathionylspermidine synthetase/amidase (gss) from Escherichia coli (strain K12)
KEGG orthology group: K01460, glutathionylspermidine amidase/synthetase [EC: 3.5.1.78 6.3.1.8] (inferred from 96% identity to enc:ECL_04317)MetaCyc: 83% identical to fused glutathionylspermidine amidase / glutathionylspermidine synthetase (Escherichia coli K-12 substr. MG1655)
Glutathionylspermidine synthase. [EC: 6.3.1.8, 6.3.1.9]; Glutathionylspermidine amidase. [EC: 6.3.1.8, 6.3.1.9, 3.5.1.78]
Predicted SEED Role
"Glutathionylspermidine synthase (EC 6.3.1.8) / Glutathionylspermidine amidohydrolase (EC 3.5.1.78)" in subsystem Glutathionylspermidine and Trypanothione (EC 3.5.1.78, EC 6.3.1.8)
MetaCyc Pathways
- glutathionylspermidine biosynthesis (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.8
Use Curated BLAST to search for 3.5.1.78 or 6.3.1.8 or 6.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (620 amino acids)
>MPMX20_03841 Bifunctional glutathionylspermidine synthetase/amidase (Enterobacter sp. TBS_079) MRKGKLSSDAPFGTLLGYAPGGVAIYSSNYGSLDPRRYPEDADFRSYIGNEYMGHKWQCV EFARRFLFLNYGCVFTDVGMAWEIFSLRFLRQVVNDNILPLQAFANGSKRAPQAGALLIW QKGGEFHETGHVAVIAQLLDDKVRIAEQNVIHSPLPLGQQWTRELRMTVENGTYTLHDTF NDTEILGWMIQTDDTEHSIPQPELDGELLKISGARLKNKRQFDGKWLNENDALQQAYVRA NGHFINKDPCQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPR LRLSWQWRRHHMITGRMDFCMDERGIKVYEYNADSASCHTEGGLILEEWVKNGYRGNGHN PAEGLLEELTGAWKHSHARPFVHIMQDNDVEEDYHALFIQRSLLQAGFETKILHGLNALS WDAAGQLIDDEGRHVNCVWKTWAWETAIEQIREVSETEYAAVPIRTGHPAGDVRLIDVLL RPEVLVFEPLWTVIPGNKAILPVLWQLFPNHRYLLDTDFEVNDLLKQTGYAIKPIAGRCG SNIDLISAGDEVLDKSSGKFVDRKNIYQQLWCLPKVDGKHIQVCTFTVGGNYGGTCLRGD DSLVVKKESDIEPLIVVKDK