Protein Info for MPMX20_03824 in Enterobacter sp. TBS_079
Annotation: Citrate lyase alpha chain
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to CILA_KLEPN: Citrate lyase alpha chain (citF) from Klebsiella pneumoniae
KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 96% identity to enc:ECL_04295)MetaCyc: 68% identical to citrate lyase alpha subunit (Klebsiella pneumoniae)
Citrate (pro-3S)-lyase. [EC: 4.1.3.6]; Citrate CoA-transferase. [EC: 4.1.3.6, 2.8.3.10]
Predicted SEED Role
"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)
MetaCyc Pathways
- citrate degradation (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.6
Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (505 amino acids)
>MPMX20_03824 Citrate lyase alpha chain (Enterobacter sp. TBS_079) MNQTELLHVNFPHLRDLKPFDAAHSATPWLNSPDEKHTRKLCASVEEAVLRSGLQDGMTV SFHHAFREGDRVINTVVALLARMGFKNLTLASSSLMTCNDALIEHIESGVITRIYTSGMR GKLADAISHGLMAEPVQIHSHGGRVKLLQDGELNIDVAFLGVPCSDEFGNANGTHGKSCC GSLGYAMVDAHFARKVVLLTEALVPFPNMPASLVQDQVDYIVQVESVGDPAKISVGAARV TSNPRELMIARYAADVIEHSGYFKPGFSMQTGSGAAATACTRFMEEKMERSGVKARFALG GITGSLVDLHEKGLIEKLLDTQCFDGEAAASLARNPNHVEISTNVYANPGSKAASCDQLD VVILSALEIDVDFNVNVITGSDGVMRGASGGHCDVAAAANLTIVVAPLLRSRIPTVVKRV TTRLTPGESIDVLVTDHGIAVNPARPEIRERLLAAGLNVVDINALFERAISLTGVPKPIE FTDKIVGVIRYRDGSVIDTVRQVKE