Protein Info for MPMX20_03716 in Enterobacter sp. TBS_079
Annotation: 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to KDUI_ECOHS: 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) from Escherichia coli O9:H4 (strain HS)
KEGG orthology group: K01815, 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase [EC: 5.3.1.17] (inferred from 96% identity to enc:ECL_04176)MetaCyc: 94% identical to 5-dehydro-4-deoxy-D-glucuronate isomerase (Escherichia coli K-12 substr. MG1655)
Glucose-6-phosphate isomerase. [EC: 5.3.1.9]; 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase. [EC: 5.3.1.9, 5.3.1.17]
Predicted SEED Role
"4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 5.3.1.17)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (17/17 steps found)
- gluconeogenesis I (13/13 steps found)
- glycolysis I (from glucose 6-phosphate) (13/13 steps found)
- superpathway of anaerobic sucrose degradation (17/19 steps found)
- colanic acid building blocks biosynthesis (11/11 steps found)
- glycolysis III (from glucose) (11/11 steps found)
- homolactic fermentation (11/12 steps found)
- heterolactic fermentation (15/18 steps found)
- 4-deoxy-L-threo-hex-4-enopyranuronate degradation (5/5 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
- Bifidobacterium shunt (12/15 steps found)
- GDP-mannose biosynthesis (4/4 steps found)
- sucrose degradation III (sucrose invertase) (4/4 steps found)
- sucrose degradation IV (sucrose phosphorylase) (4/4 steps found)
- 1,3-propanediol biosynthesis (engineered) (7/9 steps found)
- sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
- sucrose degradation II (sucrose synthase) (4/5 steps found)
- gluconeogenesis III (9/12 steps found)
- sucrose biosynthesis II (6/8 steps found)
- O-antigen building blocks biosynthesis (E. coli) (8/11 steps found)
- UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
- D-sorbitol biosynthesis I (2/3 steps found)
- glycolysis V (Pyrococcus) (7/10 steps found)
- UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
- chitin biosynthesis (6/9 steps found)
- 1,5-anhydrofructose degradation (3/5 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- sucrose biosynthesis III (1/3 steps found)
- starch biosynthesis (6/10 steps found)
- formaldehyde oxidation I (3/6 steps found)
- UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (19/31 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
- superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Pentose and glucuronate interconversions
- Pentose phosphate pathway
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.17, 5.3.1.9
Use Curated BLAST to search for 5.3.1.17 or 5.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (278 amino acids)
>MPMX20_03716 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (Enterobacter sp. TBS_079) MDVRQSIHSAHAKTLDTQGLRNEFLVEQVFEADKYTMVYSHIDRIIIGGIMPVTQTVSVG GEVGKQLGVSYFLERRELGVINIGGPGTITVDGQCYEIGHRDALYVGKGAKEVVFASIDS GKPAKFYYNCAPAHTPYPTKKVTPADVAPVTLGDNLTSNRRTINKYFVPDVLETCQLSMG LTELAPGNLWNTMPCHTHERRMEVYFYFNMDEDACVFHMMGQPQETRHIVMHNEQAVISP SWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVNELR