Protein Info for MPMX20_03684 in Enterobacter sp. TBS_079

Annotation: Protease 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 960 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00675: Peptidase_M16" amino acids 55 to 191 (137 residues), 126.7 bits, see alignment E=1.1e-40 PF05193: Peptidase_M16_C" amino acids 215 to 393 (179 residues), 79.2 bits, see alignment E=5.7e-26 amino acids 681 to 857 (177 residues), 64.4 bits, see alignment E=2e-21 PF16187: Peptidase_M16_M" amino acids 400 to 674 (275 residues), 185.2 bits, see alignment E=2.2e-58

Best Hits

Swiss-Prot: 84% identical to PTRA_SHIFL: Protease 3 (ptrA) from Shigella flexneri

KEGG orthology group: K01407, protease III [EC: 3.4.24.55] (inferred from 97% identity to enc:ECL_04144)

MetaCyc: 84% identical to protease 3 (Escherichia coli K-12 substr. MG1655)
Pitrilysin. [EC: 3.4.24.55]

Predicted SEED Role

"Protease III precursor (EC 3.4.24.55)" (EC 3.4.24.55)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (960 amino acids)

>MPMX20_03684 Protease 3 (Enterobacter sp. TBS_079)
MPGSTLFKAFVLFVALWAPFTQAGSGWQPIQETIRKSEKDTRQYQAIRLDNGMTVLLVSD
PQAVKSLSALVVPVGSLEDPEAHPGLAHYLEHMTLMGSKKYPQPDSLSEFLKMHGGSHNA
STAPYRTAFYLEVENDALGGAVDRLADSIAAPLLDKKYADRERNAVNAELTLARTRDGMR
MAQVSAETINPAHPGSRFSGGNLETLSDKPNSPVLDALHAFRDKYYSANLMKAVIYSNKP
LPELASMAAQTFGRVPNKNIDLPQINVPVVTDAQKGIVIHYVPALPRKVLRVEFRIDNNT
AQFRSKTDELVTYLIGNRSPGTLSDWLQKQGLVEGIRADSDPVVNGNSGVLAISATLTDK
GLAHRDDVVAAIFSYLSLLRDKGVDKRYFDELAHVLDLDFRYPSITRDMDYVEWLADTMI
RVPVEHTLDAVNIADQYDADAVKARLAMMTPQNARVWYISPNEPHNKMAYFVDAPYQVDK
ISDQTFADWQKKAGEIALKLPELNPYIPDDFSLIKTTKAYPHPELIVDEPTLRVVYTPSR
YFTSEPKADVSVVLRNPKAMNSAKNQVMFALNDYLAGIALDQLSNQAAVGGISFSTNANN
GLMVNANGYTQRLPQLFQALLEGYFSYTPTEEQLEQAKSWYAQMMDSAEKGKAYDLALMP
TQMLSQIPYFQREDRRALLPSITLKDVLAYRDALKTNTRPEFLIVGNMSDVQAKTLAENV
RTQLGSKGDEWCRNQDVLVEKKQNVIFEKAGNSTDSALAAVFVPTGYDEFTSTAQSAVLG
QIIQPWFYNQLRTEEQLGYAVFAFSMNVGRQWGLGFLLQSSDKQPAYLWQRYQAFFPQAE
AKLRAMKPEEFAQIQQAVIAQVMQPPQTLGEEASQLSKDFDRGNLKFDSRDKVVAEIKQL
TPQKVADFFHQAVVKPQGMAILSQVSGSQNGKTDYVNPKGWTIWKSVSALQQSMPWSKKE