Protein Info for MPMX20_03639 in Enterobacter sp. TBS_079

Annotation: Siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 PF13241: NAD_binding_7" amino acids 6 to 96 (91 residues), 78.9 bits, see alignment E=7.8e-26 TIGR01470: siroheme synthase, N-terminal domain" amino acids 43 to 188 (146 residues), 187.9 bits, see alignment E=1.7e-59 PF14824: Sirohm_synth_M" amino acids 100 to 126 (27 residues), 32.8 bits, see alignment (E = 7.8e-12) PF10414: CysG_dimeriser" amino acids 131 to 188 (58 residues), 63.3 bits, see alignment 2.9e-21 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 196 to 429 (234 residues), 300 bits, see alignment E=1.4e-93 PF00590: TP_methylase" amino acids 198 to 408 (211 residues), 187.4 bits, see alignment E=6.8e-59

Best Hits

Swiss-Prot: 76% identical to CYSG1_KLEP7: Siroheme synthase 1 (cysG1) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 95% identity to enc:ECL_04102)

MetaCyc: 54% identical to uroporphyrinogen-III C-methyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Sirohydrochlorin ferrochelatase. [EC: 4.99.1.4]; Uroporphyrinogen-III C-methyltransferase. [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; Precorrin-2 dehydrogenase. [EC: 4.99.1.4, 2.1.1.107, 1.3.1.76]

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (452 amino acids)

>MPMX20_03639 Siroheme synthase (Enterobacter sp. TBS_079)
MDYLPLFAAIKDRPVLVVGTGEVADRKIAFLQRAGAQVRVVDEADFDESQIDTVVLVIAA
TDNRELNRRISEAAQARHRLVNVVDDRPLCSFIFPSIVDRSPLLVAISSGGTAPVLARVL
REKIEALLPTSLGRMAEKASFWRNHLKTRLTSVTERRRFWERVFRGRFASLMHAGNETAA
QQILEDELDNPGSSGGEIILVGAGPGDAGLLTLRGLQVLQDADVVFYDHLVTDGVRELIR
RDAEQICVGKRAGEHSVPQHDTNQMLVAAAKAGKTVVRLKGGDPFIFGRGGEELQAAAEA
GVPFQVVPGITAASAVTAYAGIPLTHRDYAQSVTFVTGHYKADSTPFDWSHLAQSRQTLA
IYMGTMKAADISEQLIQHGREATTPVAVISRGTRVDQHVAIGTLQDLATLAKEAPMPALI
VVGEVVHLHSTLAWFQHTTDSEGFGSSVVNLA