Protein Info for MPMX20_03565 in Enterobacter sp. TBS_079

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 549 (545 residues), 780.6 bits, see alignment E=4.4e-239 PF00682: HMGL-like" amino acids 31 to 311 (281 residues), 246.7 bits, see alignment E=4.2e-77 PF22615: IPMS_D2" amino acids 329 to 391 (63 residues), 101.4 bits, see alignment E=4.1e-33 PF08502: LeuA_dimer" amino acids 420 to 548 (129 residues), 73.4 bits, see alignment E=2.6e-24

Best Hits

Swiss-Prot: 63% identical to LEU1_PSEU5: 2-isopropylmalate synthase (leuA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 93% identity to enc:ECL_04024)

MetaCyc: 44% identical to 2-(4-hydroxybenzyl)-malate synthase (Aspergillus rugulosus)
2.3.3.-

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (549 amino acids)

>MPMX20_03565 2-isopropylmalate synthase (Enterobacter sp. TBS_079)
MLKTPSDKYQPQPAISLPDRRWPEQRLTQAPRWLSTDLRDGNQALAEPMDSSRKLQFWAL
LLRCGFKEIEVAFPAASQTDFNFVRQLIDQNRIPDDVTIQVLTQAREDLILRTFASLRGA
KQATVHLYNATAPLFRRLVFGMEKAQIVELATRSTRLIRTLCEENPATRWQYEYSPETFC
FTEPDFALEICEAVADIWQPCAERPMVINLPATVEVSTPNVYADQIEYFCRHFSRRSDVC
ISVHPHNDRGTGVASAELALMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGISPNLD
FSDMNHVVEVVETCNQLPVHPRHPWAGRLAYTAFSGSHQDAIKKGFDARKPDERWEMPYL
PVDPQDIGCTYEAVIRVNSQSGKSGSAWLIEQNHGLKLPRALQQDFSQHVQQETDQHGKE
MTQHALWQLFRTRYGLVETPGFALHAYRSDSQQDGQLHLTASVTTQGGMRQLEGQGNGLL
SAAANGLSRWVTTPFVIKDYHEHTLGERSDSRSVAYIRCLFQDGSSRWGVGIDSDVARAS
LQALFNAVS