Protein Info for MPMX20_03462 in Enterobacter sp. TBS_079

Annotation: Polyphenol oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02578: Cu-oxidase_4
  • amino acids 20 to 241 (222 residues), 265.7 bits, E=1.8e-83
• TIGR00726: YfiH family protein
  • amino acids 26 to 242 (217 residues), 254.6 bits, E=3.3e-80

Best Hits

Swiss-Prot: 84% identical to POLOX_ECOLI: Polyphenol oxidase (yfiH) from Escherichia coli (strain K12)

KEGG orthology group: K05810, conserved hypothetical protein (inferred from 93% identity to enc:ECL_03925)

MetaCyc: 84% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]

Predicted SEED Role

"COG1496: Uncharacterized conserved protein"

MetaCyc Pathways

• superpathway of purine nucleotide salvage (13/14 steps found)
• L-methionine salvage cycle III (10/11 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• ureide biosynthesis (6/7 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• 5'-deoxyadenosine degradation I (2/3 steps found)
• adenosine nucleotides degradation II (3/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• sesamin biosynthesis (1/8 steps found)
• justicidin B biosynthesis (1/10 steps found)
• matairesinol biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1, 2.4.2.28, 3.5.4.4

Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4

Protein Sequence (243 amino acids)

>MPMX20_03462 Polyphenol oxidase (Enterobacter sp. TBS_079)
MTKLIVPEWPLPEGVAACSSTRIGGVSQGAWESLNLGAHCGDNLEHVEENRKRLFAAGNL
PSKPVWLEQVHGKTVLTLTGEPYASKRADASYSDTPGTVCAVMTADCLPVLFCNRAGTEV
AAAHAGWRGLCEGVLEETVACFKDDPANIMAWLGPAIGPRAFEVGPEVREAFMEKDLQAA
DAFLPAGEKYLADIYLLARQRLNNVGVTQIFGGDRCTFTEKGDFFSYRRDKTTGRMASFI
WLI