Protein Info for MPMX20_03440 in Enterobacter sp. TBS_079

Annotation: Elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 2 to 594 (593 residues), 1000.7 bits, E=2.1e-305
• PF00009: GTP_EFTU
  • amino acids 2 to 181 (180 residues), 185 bits, E=2.3e-58
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 172 (168 residues), 58.2 bits, E=8.4e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 42.3 bits, E=1.7e-14
• PF00679: EFG_C
  • amino acids 399 to 481 (83 residues), 69.6 bits, E=3.9e-23
• PF06421: LepA_C
  • amino acids 487 to 592 (106 residues), 167.5 bits, E=1.7e-53

Best Hits

Swiss-Prot: 98% identical to LEPA_ENT38: Elongation factor 4 (lepA) from Enterobacter sp. (strain 638)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 99% identity to enc:ECL_03907)

MetaCyc: 94% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (599 amino acids)

>MPMX20_03440 Elongation factor 4 (Enterobacter sp. TBS_079)
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
LDYKAADGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVTDVLERLVRDI
PAPEGDPDAPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDILGAPVGDTLTGARNPADKALPGFKKVKPQVYAGLF
PVSSDDYENFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
LDLITTAPTVVYEVQTTNKEIVYVDSPSKLPPLNNIEELREPIAECHMLLPQEFLGNVIT
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKELIPRQQFDIAIQAAIGNHIIARS
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK