Protein Info for MPMX20_03279 in Enterobacter sp. TBS_079

Annotation: Sensor histidine kinase YpdA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 signal peptide" amino acids 1 to 8 (8 residues), see Phobius details amino acids 25 to 25 (1 residues), see Phobius details transmembrane" amino acids 9 to 24 (16 residues), see Phobius details amino acids 39 to 58 (20 residues), see Phobius details amino acids 69 to 91 (23 residues), see Phobius details amino acids 97 to 122 (26 residues), see Phobius details amino acids 134 to 157 (24 residues), see Phobius details amino acids 167 to 185 (19 residues), see Phobius details PF07694: 5TM-5TMR_LYT" amino acids 22 to 186 (165 residues), 148 bits, see alignment E=5e-47 PF07155: ECF-ribofla_trS" amino acids 40 to 177 (138 residues), 29.4 bits, see alignment E=1.9e-10 PF01590: GAF" amino acids 221 to 339 (119 residues), 26.6 bits, see alignment E=2e-09 PF06580: His_kinase" amino acids 353 to 432 (80 residues), 88.7 bits, see alignment E=6.3e-29 PF02518: HATPase_c" amino acids 450 to 547 (98 residues), 42.9 bits, see alignment E=1.5e-14

Best Hits

Swiss-Prot: 92% identical to YPDA_ECO57: Sensor histidine kinase YpdA (ypdA) from Escherichia coli O157:H7

KEGG orthology group: K02478, two-component system, LytT family, sensor kinase [EC: 2.7.13.3] (inferred from 97% identity to enc:ECL_03724)

MetaCyc: 92% identical to sensor histidine kinase PyrS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Autolysis histidine kinase LytS" in subsystem Murein hydrolase regulation and cell death

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (559 amino acids)

>MPMX20_03279 Sensor histidine kinase YpdA (Enterobacter sp. TBS_079)
MLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKELLAVTAIFSLFALFSTWSGVPV
EGSLVNVRIIAVMSGGILFGPWVGVITGLIAGTHRYLIDIGGVTAIPCFITSIIAGVLSG
WINRKIPKKQRWRAGIIAGMVCETLTMILVVVWAPTIALGLDIVSKIGIPMILGSVCIGF
IVLLVQSVEGEKEASAARQAKLALDIANKTLPLFRHVNAESLRQVCDIIRRDIHADAVAI
TNNDHVLAYVGVGEHNYRDNDDTISPTTRQAINYGKIIIKNNDEAHRTPEIHSMLVIPLW
EKGVVTGTLKIYYCHAHQITSTLQEMAIGLSQIISTQLEVSRAEQLREMANKAELRALQS
KINPHFLFNALNAISSSIRMNPDTARQLIFNLSRYLRYNIELNDDEQIDIKKELYQIKDY
IAIEQARFGDKLTVIYDIDEEVNCVIPSLLIQPLVENAIVHGIQPCKGKGVVTISVTESG
NRVRIAVRDTGHGIDPKVIERVESNEMPGNKIGLLNVHHRVKLLFGDGLHIHRLEPGTEI
AFYVPNERSPVHAATSLLP