Protein Info for MPMX20_03213 in Enterobacter sp. TBS_079

Annotation: NADH-quinone oxidoreductase subunit G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 PF13510: Fer2_4" amino acids 3 to 77 (75 residues), 43.1 bits, see alignment E=1.1e-14 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 655 (652 residues), 679.6 bits, see alignment E=2.6e-208 PF00111: Fer2" amino acids 4 to 72 (69 residues), 31.5 bits, see alignment 4.2e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 124 (37 residues), 56.8 bits, see alignment (E = 3.8e-19) PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 45 bits, see alignment 2.5e-15 PF00384: Molybdopterin" amino acids 280 to 637 (358 residues), 57 bits, see alignment E=5.1e-19

Best Hits

Swiss-Prot: 92% identical to NUOG_SALTY: NADH-quinone oxidoreductase subunit G (nuoG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 98% identity to enc:ECL_03627)

MetaCyc: 91% identical to NADH:quinone oxidoreductase subunit G (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (908 amino acids)

>MPMX20_03213 NADH-quinone oxidoreductase subunit G (Enterobacter sp. TBS_079)
MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTR
GRLVMSCMTPATEGTFISIDDEEAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVM
TGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRYYKDYADGQDLGVYGAHDNVY
FGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRKWDMQFAPSICQQCSLGCNTSPG
ERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDFITLNAEQAMQ
GAADILRQSKKVIGIGSPRASIESNFALRELVGAENFYTGIAQGEQERLQLVLKVLREGG
IHTPALREIESYDAVLVLGEDLTQTGARAALAVRQAVKGKAREMAAAQKVADWQIAAILN
IGQRAKHPLFVTNVDNTRLDDIAAWTYCAPVEDQARLGFAIAHALDSNSPAVEGLDRDLQ
NKVDVIVQALAGAKKPLIVSGTNAGSAEIIQAAANVAKALKGRGADVGVTMIARAVNSIG
LGMIGGGSLEAALSELESGAADAVVVLENDLHRHASAARVDAALSKAPLVMVIDHQRTAI
MDKAHLVLSAASFAESDGTVINNEGRAQRFFQVYDPAYYDSNTIMLESWRWLHSLHSTVQ
SREVDWTQLDHVIDAVVEKLPQLAGIKDAAPDASFRIRGQKLAREPHRYSGRTAMRANIS
VHEPRQPQDKDTMFAFSMEGNNQPSAPRSQVPFAWAPGWNSPQAWNKFQAEVGGSLRHGD
PGVRLIEASNTGLDFFTTVPASFQAQEGNWRIAPYYHLFGSDELSQRSPVFQSRMPQPYI
KLNPADAAKLGVNAGANIAFSYDGQTISLPLIISESLTAGQVGLPMGMPGIAPVLAGARL
DNLQEAKA