Protein Info for MPMX20_03131 in Enterobacter sp. TBS_079

Annotation: PTS system fructose-specific EIIB'BC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 transmembrane" amino acids 231 to 256 (26 residues), see Phobius details amino acids 263 to 290 (28 residues), see Phobius details amino acids 300 to 326 (27 residues), see Phobius details amino acids 346 to 366 (21 residues), see Phobius details amino acids 378 to 399 (22 residues), see Phobius details amino acids 424 to 448 (25 residues), see Phobius details amino acids 460 to 479 (20 residues), see Phobius details amino acids 485 to 506 (22 residues), see Phobius details amino acids 515 to 546 (32 residues), see Phobius details TIGR00829: PTS system, Fru family, IIB component" amino acids 105 to 188 (84 residues), 130.2 bits, see alignment E=2.8e-42 PF02302: PTS_IIB" amino acids 106 to 190 (85 residues), 71.5 bits, see alignment E=7.4e-24 TIGR01427: PTS system, Fru family, IIC component" amino acids 212 to 549 (338 residues), 490 bits, see alignment E=4.7e-151 PF02378: PTS_EIIC" amino acids 230 to 493 (264 residues), 64.5 bits, see alignment E=9.4e-22

Best Hits

Swiss-Prot: 91% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 97% identity to enc:ECL_03470)

MetaCyc: 91% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>MPMX20_03131 PTS system fructose-specific EIIB'BC component (Enterobacter sp. TBS_079)
MKTLLIIDSGLGQARAYMAKTLLGAAANKAHLEMTDNPGDAEMVIVLGDKIPADSALNGK
KVWLGDINRAVAHPELFLSEAKGHASVYSAPVAAPVAASGPKRIVAITACPTGVAHTFMA
AEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADLVMVAADIEVDLAKFAGKPMYR
TSTGLALKKTAQEFDKALVEAKPYQATGSTQTATEGKKESAGAYRHLLTGVSYMLPMVVA
GGLCIALSFAFGITAFKEEGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTPGL
IGGMLAVSTGSGFIGGIIAGFLAGYVAKLISSKLKLPQSMEALKPILIIPLISSLVVGLA
MIYLIGKPVAGILAGLTHWLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLLS
TQTYAPMAAIMAAGMVPPLALGLATIIARRKFDKAQQEGGKAALVLGLCFITEGAIPFAA
RDPMRVLPCCIVGGAVTGAISMAVGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGTLV
AGLSYAVLKRPEAEGVAKAA