Protein Info for MPMX20_02976 in Enterobacter sp. TBS_079

Annotation: Aerobactin synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 PF04183: IucA_IucC" amino acids 130 to 372 (243 residues), 236.1 bits, see alignment E=4.2e-74 PF06276: FhuF" amino acids 394 to 549 (156 residues), 126.8 bits, see alignment E=1.2e-40

Best Hits

Swiss-Prot: 65% identical to IUCC_ECOLX: Aerobactin synthase (iucC) from Escherichia coli

KEGG orthology group: K03895, aerobactin synthetase subunit beta [EC: 6.3.2.27] (inferred from 92% identity to enc:ECL_03318)

MetaCyc: 65% identical to aerobactin synthase (Escherichia coli K-12)
RXN-13056 [EC: 6.3.2.39]

Predicted SEED Role

"Citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase, alpha subunit (EC 6.3.2.27), aerobactin biosynthesis protein IucA @ Siderophore synthetase superfamily, group C @ Siderophore synthetase component, ligase" (EC 6.3.2.27)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.27

Use Curated BLAST to search for 6.3.2.27 or 6.3.2.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (580 amino acids)

>MPMX20_02976 Aerobactin synthase (Enterobacter sp. TBS_079)
MNVYWQKVNRDMVAKILAELEYERTLSARQLADNRWQITMGAETWQFQATRGIWGWLHID
PESLTTVSGNAVDAETALLQLATVLEMSDAQTAEHLEDLYATLRGDIQLLQARETLDADA
LIHLDPDELQCLMSGHPKFIFNKGRRGWGLDALRQYAPEYRGRFRLHWVAVQRAHLVWSS
DVDCDINTLLASAMDNAERARFDARWQALDLDDNWLPVPLHPWQWQQKIAVHFLAQLARG
EMVELGEFGDEYLAQQSLRTLTNASRRAPFDIKLPLTIYNTSCYRGIPGKYIAAGPLASR
WLQQQFARDATLIRCGAQVLGEPAAGYLSHPGYAALPKAPYRYQEMLGVIWRENPSCYLQ
QGEEAVLMAALMESDNAGRPLIDAWIAQSGLTAEAWLEKLFDATVIPFYHLLCRYGVALI
AHGQNVTLVIKDAIPQRILLKDFQGDMRLVDEDFPQAASLPEQVKSVTARLSADYIIHDL
QTGNFVTVLRFISRLTLRSGVSETRFYQILAEVLRRYMAAHPDLAERFAKFDLFKPQIIR
VILNPVKLTFSEHDGGSRMLPNYVTDLDNPLFLVSQESAQ