Protein Info for MPMX20_02728 in Enterobacter sp. TBS_079

Annotation: Peptidoglycan D,D-transpeptidase FtsI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 568 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF03717: PBP_dimer" amino acids 56 to 210 (155 residues), 71 bits, see alignment E=1.8e-23 PF00905: Transpeptidase" amino acids 249 to 543 (295 residues), 261.7 bits, see alignment E=8.8e-82

Best Hits

Swiss-Prot: 64% identical to FTSI_ECOLI: Peptidoglycan D,D-transpeptidase FtsI (ftsI) from Escherichia coli (strain K12)

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 95% identity to enc:ECL_01481)

MetaCyc: 64% identical to peptidoglycan DD-transpeptidase FtsI (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (568 amino acids)

>MPMX20_02728 Peptidoglycan D,D-transpeptidase FtsI (Enterobacter sp. TBS_079)
MITTAGNFTPARFALLCLAIFCSLAFLLGRVAWLQIIKPDNLVKQEDMRSLRELPIDAPR
GMIMDREGRPLAVSVPVRAVWADPKTVLAKGGVGFDDRWQALANALHLSLNTLSSRINAN
PQGRFIYLARQVDPAQAKWIDKLNLPGINLRDESRRFYPAGHVAANLIGFTNIDGQGIEG
VEKSFNTQLTGKPGVRQVREDRYGRVVENLTEVAPVPAHNIQLSIDERLQTITEDALDNA
VAWNKAESGASVLINIPTGEILAMASYPDFNPNNRQGATIDDFRNRAISDTFEPGSTVKP
LVLMTALQQGLVQPDSVIDTHPYMLDGHRIRDVGYYPELSMTGILQKSSDTGVSRLSLAM
PVQHLIDTYRNFGFGTNTGLGLTGESAGLLPQRKYWSQLDRATFAFGYGLMVTPLQLAHV
YATIGGFGLERPLSITRIDPPVIGHRVMPEEIAHQVEHMMESVALPGGGGVKAAVRDYRV
AVKTGTAKKIDDSGKYVDKYVAYTAGVAPASDPRFALVVVINDPQNGAYYGGAVSAPVFS
EIMGNVLRLENVKPDGLAPDSSHLIVMR