Protein Info for MPMX20_02624 in Enterobacter sp. TBS_079

Annotation: Nitrate/nitrite binding protein NrtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF13379: NMT1_2" amino acids 35 to 286 (252 residues), 349.8 bits, see alignment E=1.1e-108

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 90% identity to ent:Ent638_2325)

Predicted SEED Role

"Nitrate ABC transporter, nitrate-binding protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (415 amino acids)

>MPMX20_02624 Nitrate/nitrite binding protein NrtA (Enterobacter sp. TBS_079)
MADLSRRRFLQASMLASGAMLLPGVMQAAWAAGSDKPEQETVRIGFIPLTDCAPVVIAAL
KGFDKKYGITIVPTKEASWAAVRDKLVAGELDAAHILYGLLYGLELGLAGKPQQMANLMT
LNQNGQAITLSSDLAEKGVRDLDGLKTLIGQQAPGTYTFAHTFPTGTHAMWLYYWLASAG
INPFDDVRTVVVPPPQMVMNMRIGNMVGFCVGEPWNARAINDRIGFTAATSQSIWADHPE
KILGTRRDWVEKNPNTARALVSAVLEAARWIDASPDNKRETAQILSRRAWLNTKELYLTG
RMLGEYDNGLGQRWQDAHPIRFFNEGAVSYPYLSDGMWFLTQFRRWGLLKSAPDYAAIAQ
RINQTSVWQDAATAVGGISTPSSPYRSSTLMDGTVWNGTDPDGYANRFAIHRKGA