Protein Info for MPMX20_02329 in Enterobacter sp. TBS_079

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 595 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 20 to 562 (543 residues), 706.5 bits, see alignment E=9.6e-217 PF00128: Alpha-amylase" amino acids 127 to 210 (84 residues), 52.7 bits, see alignment E=5.1e-18 amino acids 232 to 440 (209 residues), 31.9 bits, see alignment E=1.1e-11 PF11941: DUF3459" amino acids 503 to 586 (84 residues), 49 bits, see alignment E=6.6e-17

Best Hits

KEGG orthology group: None (inferred from 87% identity to enc:ECL_01949)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (595 amino acids)

>MPMX20_02329 1,4-alpha-glucan branching enzyme GlgB (Enterobacter sp. TBS_079)
MEFRTFQKHWGAEFISNDVVRFRVWAEGQKELTLRLAENDLPMTAVGSGWFQIDVPGVTH
GTEYQYVLQDGMAVPDPASRAQAGDVNGPSVVIDARRYPPGNREWAGRPWEDTVIYELHI
GTFTPEGTFRAAIEKLPYLAELGITQIEVMPVSQFGGSRGWGYDGVLLYAPHAAYGTPED
FHAFIDAAHGLGLSVVLDIVLNHFGPEGNYLPVLSPAFFDAERMTPWGNGIAYETEPVRQ
YITEAPLFWLTEYHLDGLRFDAIDQIEDASDKHILQEIAERIREAIPGRQIHLTTEDSRN
VIFLHPRDAHGATPLFTAEWNDDFHNAAHVFATGETHAYYQDFAFEPEKKLARALTEGFV
YQGEVSLQTGKSRGVECHTQPPQFFVDFIQNHDQTGNRAQGERLIALAGADKTRVLLAAL
LLSPHIPLLFMGEEFGETNPFLFFTDFHGELAKAVREGRAKEFTGHAGHDASVPDPNDVK
TFIRSKIDWNKVATDDGKTWLRFTRTLLTLRHRHIVPLLRKGVTVEGKVIRTAPGMIAVS
WRFPTGTLSLAFNTGNKAVDVPALAGETLFSWPEVNEVLPPNSIVVRFADGEASL