Protein Info for MPMX20_02316 in Enterobacter sp. TBS_079

Annotation: Cation efflux system protein CusB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF19335: HMBD" amino acids 47 to 74 (28 residues), 45.7 bits, see alignment (E = 1e-15) PF16576: HlyD_D23" amino acids 112 to 318 (207 residues), 201.6 bits, see alignment E=2e-63 PF16572: HlyD_D4" amino acids 155 to 207 (53 residues), 69.1 bits, see alignment 4.6e-23 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 205 to 396 (192 residues), 127.3 bits, see alignment E=3.3e-41 PF13437: HlyD_3" amino acids 214 to 315 (102 residues), 50.1 bits, see alignment E=8.2e-17

Best Hits

Swiss-Prot: 85% identical to SILB_SALTM: Putative membrane fusion protein SilB (silB) from Salmonella typhimurium

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 86% identity to kpu:pK2044_00620)

MetaCyc: 69% identical to copper/silver export system membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>MPMX20_02316 Cation efflux system protein CusB (Enterobacter sp. TBS_079)
MASLRIKFAAVIIGSLIAGGLISVIAWQSVYPAEKTEQTTTERKVLFWYDPMKPDTRFDK
PGKSPFMDMDLVPKYADENDEKSSEGIRIDPTQVQNLGLKTQKVTRGALNYAQTIPANVS
YNEYQFVIVQARSEGFVERVYPLTVGDHVKKGTPLIDITIREWVEAQSEFLLLSGTGGTP
TQIKGVLERLRLAGMPEEDVQRLRATRSIQTRFTIKAPIDGVITAFNLRTGMNITKDNVV
AQIQGMDPVWISAAVPESIAYLLKDSSQFEISVPAYPDKSFPIEKWNILPSADQTTRTLQ
VRLQVSNKDESLKPGMNAYLKLNASSQEMLLIPSQAVIDTGKEQRVIVVDAEGRFVPKSI
HVLHESQQQSAIGSGLNEGDAVVVSGLFLIDSEANISGALERMRHPEKTDDAHSGH