Protein Info for MPMX20_02084 in Enterobacter sp. TBS_079

Annotation: Putative dimethyl sulfoxide reductase chain YnfE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 10 to 812 (803 residues), 1344 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 54 to 112 (59 residues), 47.7 bits, see alignment 1.8e-16 PF00384: Molybdopterin" amino acids 115 to 570 (456 residues), 234.3 bits, see alignment E=3.2e-73 PF01568: Molydop_binding" amino acids 691 to 804 (114 residues), 91.6 bits, see alignment E=4.9e-30

Best Hits

Swiss-Prot: 84% identical to YNFE_ECOLI: Putative dimethyl sulfoxide reductase chain YnfE (ynfE) from Escherichia coli (strain K12)

KEGG orthology group: K07309, Tat-targeted selenate reductase subunit YnfE [EC: 1.97.1.9] (inferred from 95% identity to enc:ECL_02189)

MetaCyc: 84% identical to putative selenate reductase YnfE (Escherichia coli K-12 substr. MG1655)
Selenate reductase. [EC: 1.97.1.9]

Predicted SEED Role

"Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3)" (EC 1.8.5.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.5.3

Use Curated BLAST to search for 1.8.5.3 or 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (812 amino acids)

>MPMX20_02084 Putative dimethyl sulfoxide reductase chain YnfE (Enterobacter sp. TBS_079)
MSDVENHGGISRRTLVKSTAISSLALAAGGISLPFGLKRAAAAVQNAVKPAEDKVVWGAC
SVNCGSRCALRLHVRDEEVYWVETDNTGEDIYGNHQVRACLRGRSIRRRINHPDRLNYPM
KRVGKRGEGKFERISWDEALDTLTRSLKDVVEKYGNEAVYINYSSGIVGGNITRSSPYAS
LVARLMNCYGGFLSHYGTYSTAQIACAMPYTYGSNDGNSTSDIENTKLVVMFGNNPAETR
MSGGGITYYLEQARERSHARMIVIDPRYTDTAAGREDEWIPIRPGTDAALVAGIAWVLID
ENLVDQPFLDTYCVGYDEKTLPEGAPANGHYKAYILGQGDDGIAKTPEWASRITGIPADR
IIKLAREIGSTKPAYICQGWGPQRQANGELTSRAIAMLPILTGNVGINGGNSGARESTYT
ITIERMPLPDNPVKTQISCFSWTDAIARGPEMTALRDGVRGKEKLDVPIKFIWNYAGNTI
INQHSDINKTHDILQDESKCETIVVIDNFMTSSAKYADILLPDLMTVEQEDIIPNDYAGN
MGYLIFLQPVTAPKFERKPIYWIMSEVAKRLGPDIHQRFTEGRTQAQWLQYLYAKMLEKD
PQLPSYDGLKERGIYKRKDPAGHFVAYKKFRDDPQAHPLNTPSGKIEIYSSKLAEIAATW
ELADDETISPLPVYASTFDGWDAPERHKFPLQLFGFHFKARTHSSYGNVDVLKAACRQEV
WLNPVDAAQRGIKNGDTVRVFNDRGEVRIEAKVTPRIMPGVSAMGQGAWHDANMNGDRVD
HGSCINTLTTHRPSPLAKGNPQHTNLVQIEKA