Protein Info for MPMX20_02021 in Enterobacter sp. TBS_079

Annotation: Fumarate hydratase class I, aerobic

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05681: Fumerase
  • amino acids 51 to 326 (276 residues), 269.7 bits, E=2.7e-84
• TIGR00722: hydrolyase, tartrate alpha subunit/fumarate domain protein, Fe-S type
  • amino acids 78 to 325 (248 residues), 107.2 bits, E=8.6e-35
• PF05683: Fumerase_C
  • amino acids 332 to 537 (206 residues), 293.2 bits, E=8.6e-92
• TIGR00723: hydrolyase, tartrate beta subunit/fumarate domain protein, Fe-S type
  • amino acids 373 to 537 (165 residues), 204.8 bits, E=9.1e-65

Best Hits

Swiss-Prot: 93% identical to FUMA_ECOL6: Fumarate hydratase class I, aerobic (fumA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01676, fumarate hydratase, class I [EC: 4.2.1.2] (inferred from 98% identity to enc:ECL_02274)

MetaCyc: 93% identical to fumarase A (Escherichia coli K-12 substr. MG1655)
Oxaloacetate tautomerase. [EC: 5.3.2.2]; Fumarate hydratase. [EC: 5.3.2.2, 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class I, aerobic (EC 4.2.1.2)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• mixed acid fermentation (16/16 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VI (Helicobacter) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (7/9 steps found)
• reductive TCA cycle I (8/11 steps found)
• incomplete reductive TCA cycle (5/7 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• methylaspartate cycle (8/19 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2 or 5.3.2.2

Protein Sequence (548 amino acids)

>MPMX20_02021 Fumarate hydratase class I, aerobic (Enterobacter sp. TBS_079)
MSNKPFHYQDPFPLSKDQTEYYLLTRDHVSVSEFEGQEILKVDPQALTLLAQQAFHDASF
MLRPAHQQQVADILSDPQASENDKYVALQFLRNSDIAAKGILPTCQDTGTAIIVGKKGQR
VWTGGGDEAALAHGVYNTYTEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKF
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAES
TLKTVKLASTKYYDGLPTEGNEHGQAFRDVQLEQELLEEARNLGLGAQFGGKYFAHDVRV
IRLPRHGASCPVGMGVSCSADRNIKAKINREGVWIEKLENNPGKYIPEALRKAGEGEAVH
VDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERLDNGEGLPQYIKDHPIY
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQANGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQGSIKSLECIEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFRQIQA
SQCSACVK