Protein Info for MPMX20_01702 in Enterobacter sp. TBS_079

Annotation: Bifunctional protein PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 74.7 bits, see alignment (E = 8e-25)
• PF18327: PRODH
  • amino acids 89 to 136 (48 residues), 57.5 bits, see alignment (E = 3.2e-19)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 148 to 259 (112 residues), 155.5 bits, E=1.8e-49
• PF01619: Pro_dh
  • amino acids 268 to 569 (302 residues), 359.9 bits, E=2.8e-111
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 610 to 1112 (503 residues), 880.3 bits, E=1.7e-269
• PF00171: Aldedh
  • amino acids 659 to 1103 (445 residues), 367.7 bits, E=1.9e-113

Best Hits

Swiss-Prot: 90% identical to PUTA_ECOLI: Bifunctional protein PutA (putA) from Escherichia coli (strain K12)

MetaCyc: 89% identical to trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Salmonella enterica enterica serovar Typhimurium)
RXN-14903 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-proline degradation I (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Protein Sequence (1320 amino acids)

>MPMX20_01702 Bifunctional protein PutA (Enterobacter sp. TBS_079)
MGMTTMGVKLDDATRERIKTAATRIDRTPHWLIKQAIFNYLERLESDEGLPELPALLAGA
ANESDEVSAAADENHQPFLEFAEQILPQSVSRAAITGAYRRAETDAVPMLLEQARLPEAI
ASQAHSLAYQLADKLRNQKNATGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKAT
RDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGRLVSTHNEANLSRSLNRIIGKSG
EPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEDKGFRYSYDMLGEAALTAADAQAY
MVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLA
RQYDIGINIDAEEADRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPFVIDYLIDL
ASRSRRRLMIRLVKGAYWDSEIKRAQMEGLEGYPVYTRKVYTDVSYLACAKKLLGVPNLI
YPQFATHNAHTLAAIYSLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIY
APVGTHETLLAYLVRRLLENGANTSFVNRIADTTLPLDELVADPVQAVEKMAAQEGQIGL
PHPKIPLPRELYGKGRVNSAGLDLANEHRLASLSSALLNSALQKWQAKPILEQPVADGEM
QPVINPAEPKDIVGYVREATEAEVDQALDSAVNNAPIWFATPPQERGAILERAAVLMEDQ
MQSLIGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRDDFDNETHRPLGPVVCISPWNF
PLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQGINILLEAGVPAGVIQLLPGRGETVGA
KLTADNRVRGVMFTGSTEVASLLQRNIATRLDAQGRPTPLIAETGGMNAMIVDSSALTEQ
VVVDVLASAFDSAGQRCSALRVLCLQDDVADHTLKMLRGAMAECRMGNPGRLTTDIGPVI
DAEAKANIENHIQTMRAKGRPVFQAVRENSEDAREWQTGTFVPPTLIELASFDELKKEVF
GPVLHVVRYNRNNLNELIEQINASGYGLTLGVHTRIDETIAQVTGSAKVGNLYVNRNMVG
AVVGVQPFGGEGLSGTGPKAGGPLYLYRLLANRPDNALGVTLARQDTERPVDAQLKAVLT
QPLEALITWAEKRPELRAVAQQYGELAQAGTQRLLPGPTGERNTWTLMPRERVLCVADNE
QDALVQLAAAAATGCEVLWPEDALHRDLAKQLPKAVSARIHFAKPDALLTQPFDAVIYHG
DSDQLRELCEQVAARSGAIVSVQGFARGETNLLLERLYVERSLSVNTAAAGGNASLMTIG