Protein Info for MPMX20_01495 in Enterobacter sp. TBS_079

Annotation: Putrescine-binding periplasmic protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 44 to 304 (261 residues), 72.7 bits, see alignment E=1.4e-23 PF13531: SBP_bac_11" amino acids 45 to 307 (263 residues), 27.9 bits, see alignment E=4.8e-10 PF13416: SBP_bac_8" amino acids 46 to 335 (290 residues), 112.2 bits, see alignment E=1e-35 PF13343: SBP_bac_6" amino acids 79 to 319 (241 residues), 74.1 bits, see alignment E=3.2e-24

Best Hits

Swiss-Prot: 91% identical to POTF_ECOLI: Putrescine-binding periplasmic protein (potF) from Escherichia coli (strain K12)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 98% identity to enc:ECL_02819)

MetaCyc: 91% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>MPMX20_01495 Putrescine-binding periplasmic protein (Enterobacter sp. TBS_079)
MIALNKKWLSGLVAGALMAVSAGTLAAEQKTLHVYNWSDYIAPDTVANFEKETGIKVVYD
VFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLDKSKLPNWKNLDPEVLKLV
AKHDPDNKYAMPYLWATTGIGYNVDKVKAVLGKDVKLDSWDVVLKPENLEKLKSCGVSFL
DAPEEIFATVLNYLGKDPNSSKADDYTGPATDLLLKLRPNIRYFHSSQYINDLANGDICV
AIGWAGDVWQAANRAKEAKNGVNVSYFIPKEGALAFFDVFAMPADAKNKDEAYQFLNYLM
RPEVIAHISDHVYYANGNKASVPLVSEEIRNNPAIYPPADVFAKLFTLKVQDPKIDRVRT
RAWTKVKSGK