Protein Info for MPMX20_01400 in Enterobacter sp. TBS

Protein Info for MPMX20_01400 in Enterobacter sp. TBS_079

Annotation: Histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 90% identical to HUTH_ENT38: Histidine ammonia-lyase (hutH) from Enterobacter sp. (strain 638)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 96% identity to enc:ECL_02964)

MetaCyc: 80% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

L-histidine degradation VI (7/8 steps found)
L-histidine degradation I (4/4 steps found)
L-histidine degradation II (4/5 steps found)
L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>MPMX20_01400 Histidine ammonia-lyase (Enterobacter sp. TBS_079)
MNALTLTPGSLTLKQLRNVWRQPVKLSLDESAHAAINDSVACVEAIIAEGRTAYGINTGF
GLLAQTRIATHDLENLQRSLVLSHAAGVGQPLDDDIVRLMMVLKINSLARGFSGIRLSVI
EALMALVNAEVYPWIPAKGSVGASGDLAPLAHMSLLLLGEGQARWKGEWLPAKEALKKAG
LTPITLAAKEGLALLNGTQASTAFALRGLFEAEDLFASAVVCGALTTEAVLGSRRPFDAR
IHEVRGQRGQIDAAAMYRHLLTETSDIADSHHNCDKVQDPYSLRCQPQVMGACLTQLRHA
AEVLLVEANAVSDNPLVFAQENEVVSGGNFHAEPVAMVADNIALAIAEVGALSERRIALM
MDKHMSQLPPFLVRNGGVNSGFMIAQVTAAALASENKALSHPHSVDSLPTSANQEDHVSM
APAAGRRLWEMAANTRGVLAVEWLAACQGVDLREGLTSSPLLEQARHLLREQVAHYDDDR
FFAPDIDKAIALLEKGSLVGLLPSVL