Protein Info for MPMX20_01300 in Enterobacter sp. TBS_079

Annotation: Leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 transmembrane" amino acids 521 to 541 (21 residues), see Phobius details TIGR00396: leucine--tRNA ligase" amino acids 5 to 859 (855 residues), 1300.3 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 225 (212 residues), 109.1 bits, see alignment E=4.6e-35 amino acids 417 to 572 (156 residues), 32.3 bits, see alignment E=9.8e-12 amino acids 618 to 652 (35 residues), 27.6 bits, see alignment (E = 2.6e-10) PF09334: tRNA-synt_1g" amino acids 39 to 183 (145 residues), 82 bits, see alignment E=7.9e-27 amino acids 607 to 659 (53 residues), 21.1 bits, see alignment 2.4e-08 PF13603: tRNA-synt_1_2" amino acids 221 to 403 (183 residues), 265.2 bits, see alignment E=5.1e-83 PF08264: Anticodon_1" amino acids 700 to 822 (123 residues), 69.4 bits, see alignment E=6.3e-23

Best Hits

Swiss-Prot: 95% identical to SYL_CITK8: Leucine--tRNA ligase (leuS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 95% identity to cko:CKO_02514)

MetaCyc: 94% identical to leucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Leucine--tRNA ligase. [EC: 6.1.1.4]; 6.1.1.4 [EC: 6.1.1.4]; 3.1.1.- [EC: 6.1.1.4]

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (860 amino acids)

>MPMX20_01300 Leucine--tRNA ligase (Enterobacter sp. TBS_079)
MQEQYRPEEIESKVQQHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
VIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYD
WSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCPNDQTVLANEQVIDGCCWRCD
TKVERKEIPQWFIKITAYADELLNDLDNLDHWPDTVKTMQRNWIGRSEGVEITFNVENDD
RTLTVYTTRPDTFMGATYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEADMATMEK
KGVDTGFKAIHPLTGEAIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFATKYGLTIKPVI
LAADGSEPDLSAQALTEKGTLFNSGEFSGLSFEDGFNAIADKLASLGVGERKVNYRLRDW
GVSRQRYWGAPIPMVTLEDGTVMPTPEDQLPVILPEDVIMDGITSPIKADPEWAKTTVNG
QPALRETDTFDTFMESSWYYARYTCPQYQEGMLDSDAANYWLPVDIYIGGIEHAIMHLLY
FRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGANGERNWVSPVDAIVERDEKGR
IVKAKDAEGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQE
SGVEGANRFLKRVWKLVYEHTSQGDAPAVNVAALTEDQQALRRDVHKTIAKVTDDIGRRQ
TFNTAIAAIMELMNKLAKAPQDGEQDRALMREALLAVVRMLNPFTPHVSFTLWQELKGEG
DIDNAPWPVADESAMVENTTLVVVQVNGKVRGKITVAVDATEEQVRERAGQEHLVAKYLE
GVTVRKVIYVPGKLLNLVVG