Protein Info for MPMX20_01228 in Enterobacter sp. TBS_079

Annotation: Oxygen-dependent choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 PF00732: GMC_oxred_N" amino acids 3 to 300 (298 residues), 209.9 bits, see alignment E=4.2e-66 TIGR01810: choline dehydrogenase" amino acids 4 to 537 (534 residues), 1018.4 bits, see alignment E=0 PF05199: GMC_oxred_C" amino acids 393 to 527 (135 residues), 145.8 bits, see alignment E=1.3e-46

Best Hits

Swiss-Prot: 95% identical to BETA_SHIF8: Oxygen-dependent choline dehydrogenase (betA) from Shigella flexneri serotype 5b (strain 8401)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 95% identity to eco:b0311)

MetaCyc: 95% identical to choline dehydrogenase (Escherichia coli K-12 substr. MG1655)
Choline dehydrogenase. [EC: 1.1.99.1]; 1.1.99.1 [EC: 1.1.99.1]; RXN0-7231 [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (554 amino acids)

>MPMX20_01228 Oxygen-dependent choline dehydrogenase (Enterobacter sp. TBS_079)
MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFRTQMPAALAFPLQGKRY
NWAYETEPEPHMNNRRMECGRGKGLGGSSLINGMCYIRGNAMDLDNWATEPGLEHWSYLN
CLPYYRKAETRDVGPNDYHGGDGPVSVTTSKPGVNPLFEAMVEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRTHAMTDHIIFDGKRATGVEWLEG
ESTIPSKATASKEVLLCAGAIASPQILQRSGVGNAELLKQFDIPLVHDLPGVGENLQDHL
EMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFEWPNI
QYHFLPVAINYNGSNAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQD
WQEFRDAIRITREIMHQPALDKYRGREISPGIECQTDEQLDEFVRNHAETAFHPCGTCKM
GYDEMAVVDGEGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADAIRGREPLAKS
TAAYYVANGAPVRL