Protein Info for MPMX20_01133 in Enterobacter sp. TBS_079

Annotation: Oxygen-insensitive NAD(P)H nitroreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00881: Nitroreductase
  • amino acids 8 to 193 (186 residues), 89 bits, E=2.1e-29

Best Hits

Swiss-Prot: 97% identical to NFSB_ENTCL: Oxygen-insensitive NAD(P)H nitroreductase (nfsB) from Enterobacter cloacae

KEGG orthology group: K10679, nitroreductase / dihydropteridine reductase [EC: 1.-.-.- 1.5.1.34] (inferred from 98% identity to enc:ECL_03157)

MetaCyc: 88% identical to NAD(P)H-dependent nitroreductase NfsB (Escherichia coli K-12 substr. MG1655)
1.7.1.-; NAD(P)H dehydrogenase (quinone). [EC: 1.6.5.2]; 6,7-dihydropteridine reductase. [EC: 1.6.5.2, 1.5.1.34]

Predicted SEED Role

"Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34)" in subsystem Pterin biosynthesis (EC 1.-.-.-, EC 1.5.1.34)

MetaCyc Pathways

• tetrahydropteridine recycling (2/2 steps found)
• L-phenylalanine degradation V (1/3 steps found)
• superpathway of photosynthetic hydrogen production (3/6 steps found)
• vitamin K-epoxide cycle (1/4 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Carotenoid biosynthesis - General
• Folate biosynthesis
• Insect hormone biosynthesis
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Puromycin biosynthesis
• Trinitrotoluene degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.6.5.2

Use Curated BLAST to search for 1.-.-.- or 1.5.1.34 or 1.6.5.2

Protein Sequence (217 amino acids)

>MPMX20_01133 Oxygen-insensitive NAD(P)H nitroreductase (Enterobacter sp. TBS_079)
MDIISVALKRHSTKAFDPSKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIIASTEEGKARV
AKSAAGTYVFNERKMMDASHVVVFCAKTAMDDAWLERVVDQEEADGRFNTPEAKAANHKG
RCYFADMHRVDLKDDDQWMAKQVYLNVGNFLLGVAALGLDAVPIEGFDAAILDEEFGLKE
KGFTSLVVVPVGHHSVEDFNATLPKSRLPLSTIVTEC