Protein Info for MPMX20_00807 in Enterobacter sp. TBS_079

Annotation: Pyruvate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00759: pyruvate dehydrogenase (acetyl-transferring), homodimeric type
  • amino acids 5 to 886 (882 residues), 1619.8 bits, E=0
• PF00456: Transketolase_N
  • amino acids 105 to 296 (192 residues), 36.4 bits, E=3.1e-13
• PF17831: PDH_E1_M
  • amino acids 475 to 700 (226 residues), 341.2 bits, E=2.5e-106

Best Hits

Swiss-Prot: 93% identical to ODP1_ECOLI: Pyruvate dehydrogenase E1 component (aceE) from Escherichia coli (strain K12)

KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 76% identity to avr:B565_0364)

MetaCyc: 93% identical to pyruvate dehydrogenase E1 component (Escherichia coli K-12 substr. MG1655)
Pyruvate dehydrogenase (acetyl-transferring). [EC: 1.2.1.104, 1.2.4.1]; 1.2.1.104 [EC: 1.2.1.104, 1.2.4.1]

Predicted SEED Role

"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (3/4 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.104 or 1.2.4.1

Protein Sequence (887 amino acids)

>MPMX20_00807 Pyruvate dehydrogenase E1 component (Enterobacter sp. TBS_079)
MSERLQNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLSEARKGGVKVASGAGASNY
VNTIAVEDEPEYPGNLDLERRIRSAIRWNAIMTVLRASKKDLELGGHMASFQSSATVYEV
CFNHFFRAANEKDGGDLVYFQGHISPGIYARAFLEGRLTEEQMNNFRQEVHGKGLSSYPH
PKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLKDTSEQTVYAFLGDGEMDEPESK
GAITIATREKLDNLCFIINCNLQRLDGPVTGNGKIINELEGIFAGAGWNVIKVMWGSRWD
ELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYVREHFFGKYPETAALVADWTDEQIWAL
NRGGHDPKKIYAALKKARETKGKATVILAHTIKGYGMGDTAEGKNIAHQVKKMNMDGVRY
IRDRFNVPVTDEQVENLSYITFPEGSEEHKYLHERRQALKGYLPARQPNFTEKLELPALE
DFSQLLEEQNKEISTTIAFVRALNVMLKNKSIKDRLVPIIADEARTFGMEGLFRQIGIYS
PNGQQYTPQDREQVAYYKEDEKGQILQEGINELGAGASWLAAATSYSTNNLPMIPFYIYY
SMFGFQRIGDLCWQAGDQQARGFLIGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYD
PSYAYEVAVIMHDGLQRMYGEAQENIYYYITTLNENYHMPAMPAGAEEGIRKGIYKLETV
AGSKGKVQLLGSGSILRHVREAAQILANDYGVGSDVYSVTSFTELARDGQDCERWNMLHP
METPRVPYIAQVMNDAPAVASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRH
HFEVDASYVVVAALGELAKRGEIDKKVVADAITKFNIDADKVNPRLA