Protein Info for MPMX20_00785 in Enterobacter sp. TBS_079

Annotation: D-alanine--D-alanine ligase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01205: D-alanine--D-alanine ligase
  • amino acids 4 to 305 (302 residues), 365 bits, E=1.4e-113
• PF01820: Dala_Dala_lig_N
  • amino acids 51 to 86 (36 residues), 44.4 bits, 4.7e-15
• PF07478: Dala_Dala_lig_C
  • amino acids 103 to 302 (200 residues), 242.3 bits, E=7e-76
• PF02786: CPSase_L_D2
  • amino acids 129 to 274 (146 residues), 31.2 bits, E=3.2e-11
• PF13535: ATP-grasp_4
  • amino acids 138 to 273 (136 residues), 30.5 bits, E=5.4e-11

Best Hits

Swiss-Prot: 86% identical to DDLB_ECOLI: D-alanine--D-alanine ligase B (ddlB) from Escherichia coli (strain K12)

KEGG orthology group: K01921, D-alanine-D-alanine ligase [EC: 6.3.2.4] (inferred from 95% identity to enc:ECL_00889)

MetaCyc: 86% identical to D-alanine--D-alanine ligase B (Escherichia coli K-12 substr. MG1655)
D-alanine--D-alanine ligase. [EC: 6.3.2.4]

Predicted SEED Role

"D-alanine--D-alanine ligase B (EC 6.3.2.4)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.4)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Alanine metabolism
• Peptidoglycan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.4

Use Curated BLAST to search for 6.3.2.4

Protein Sequence (306 amino acids)

>MPMX20_00785 D-alanine--D-alanine ligase B (Enterobacter sp. TBS_079)
MADKIAVLSGGTSAEREVSLNSGAAVLAGLREGGVDAHLVDPKETDVTQLKAMGFDKVFI
ALHGRGGEDGTLQGLLELTGMPYTGSGVMASAISMDKLRSKLLWQGAGLPVAPWVALTRR
EFEMGLSDSVNARIAELGLPVIVKPSREGSSVGMSKVEKAEDLSSALALAFQHDEEVLIE
KWLSGPEFTVAMLGEEILPSIRIQPAGTFYDYEAKYLSDETQYFCPSGLEAERETDLQSL
VVKAWHVLGCRGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVR
ILDQAG