Protein Info for MPMX20_00725 in Enterobacter sp. TBS_079

Annotation: Bifunctional riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 13 to 167 (155 residues), 188.8 bits, E=8.7e-60
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 18 to 306 (289 residues), 386.7 bits, E=3.7e-120
• PF01467: CTP_transf_like
  • amino acids 19 to 170 (152 residues), 22.6 bits, E=1.6e-08
• PF01687: Flavokinase
  • amino acids 184 to 306 (123 residues), 142.9 bits, E=9.5e-46

Best Hits

Swiss-Prot: 86% identical to RIBF_ECOLI: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli (strain K12)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 92% identity to ent:Ent638_0583)

MetaCyc: 86% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (9/9 steps found)
• flavin biosynthesis III (fungi) (8/9 steps found)
• flavin salvage (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• flavin biosynthesis II (archaea) (6/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (312 amino acids)

>MPMX20_00725 Bifunctional riboflavin kinase/FMN adenylyltransferase (Enterobacter sp. TBS_079)
MKLIRGIHNLSRAPHGCVLTIGNFDGVHRGHQALLQGLRKEGEARGLPVVVMIFEPQPLE
LFAGDKSPARLTRLREKLRYLAESGVDYVLCVRFDRRFAALTAQNFVSDLLVRRLGVQFL
AVGDDFRFGAGRQGDFLLLQKAGLEYGFDVTSAMTFCEGGVRVSSTAVRQALANDELDTA
ETLLGHPFSISGRVVHGDALGRTIGFPTANIPLRRQVSPVKGVYAVEVAGLGDKPYFGVA
NIGTRPTVAGVRQQLEVHLLDVVMDLYGRHIDVILRKKIRNEQRFASLDELKAQIARDEL
TAREFFGRVNPA