Protein Info for MPMX20_00596 in Enterobacter sp. TBS_079

Annotation: Tryptophan--tRNA ligase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 TIGR00233: tryptophan--tRNA ligase" amino acids 4 to 329 (326 residues), 210.3 bits, see alignment E=2.3e-66 PF00579: tRNA-synt_1b" amino acids 5 to 284 (280 residues), 173 bits, see alignment E=4.6e-55

Best Hits

Swiss-Prot: 54% identical to SYW_CLOLO: Tryptophan--tRNA ligase (trpS) from Clostridium longisporum

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 96% identity to enc:ECL_00704)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.2

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>MPMX20_00596 Tryptophan--tRNA ligase 2 (Enterobacter sp. TBS_079)
MNVQTILTGDRPTGPLHLGHFVGSLRQRVALQQTHNQYVLIADLQGLTDNGSNPQKIRDN
IPEVLADYLAAGIDPNLTTLCLQSALPALAELTMLYMNIVTVARVERNPTVKNEIAQKGF
ARSLPVGFMAYPISQAADITAFKAECVPVGDDQLPMIEQTNEIVHKMNSLLPAPVLRHCK
AMLSDTGRLPGIDGSAKMSKSLGNTLHLSASEETIHRAVSAMYTDPNHLKVSDPGQIEGN
VVFTYLDAFHPDKAKVAAMKAHYQAGGLGDRTCKNELEACLQELIAPMRERRAMYMQDKG
ELMAMLKRCTERAQAVTQGTLREVKIGLGVPVFS