Protein Info for MPMX20_00217 in Enterobacter sp. TBS_079

Annotation: Phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 27 to 108 (82 residues), 111.8 bits, E=1.1e-36
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 147 to 599 (453 residues), 741.9 bits, E=9.3e-228
• PF01964: ThiC_Rad_SAM
  • amino acids 148 to 595 (448 residues), 618.4 bits, E=6e-190

Best Hits

Swiss-Prot: 96% identical to THIC_CITK8: Phosphomethylpyrimidine synthase (thiC) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to enc:ECL_00255)

MetaCyc: 94% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (631 amino acids)

>MPMX20_00217 Phosphomethylpyrimidine synthase (Enterobacter sp. TBS_079)
MSTTKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGSQADIRVPMREIQLSPTLLGGSK
ENPQYEDNEAVPVYDTSGPYGDPDVAINVQQGLAKLRQRWIDARNDCEELTVRSSAYTKE
RLADDGLDELRFAGLLTPKRAKAGKCVTQLHYARQGIVTPEMEFIAIRENMGRERIRSEV
LRHQHPGEGFGARLPENITPEFVRDEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANI
GNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPVYQAL
EKVNGIAENLTWEAFRDTLLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMA
KWCLSHHQENFLYEHFREICEICAAYDVSLSLGDGLRPGSIRDANDEAQFAELHTLGELT
KIAWEYDVQVMIEGPGHVPMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGI
GAAMIGWFGCAMLCYVTPKEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAM
SKARFEFRWEDQFNLALDPFTARAYHDETLPQESGKVAHFCSMCGPKFCSMKISQEVRDY
AAAQTIEVGMADMSETFRAKGGEIYLKKEEA